TY - JOUR
T1 - Molecular phylogeny and paclitaxel screening of fungal endophytes from Taxus globosa
AU - Soca-Chafre, Giovanny
AU - Rivera-Orduña, Flor N.
AU - Hidalgo-Lara, M. Eugenia
AU - Hernandez-Rodriguez, Cesar
AU - Marsch, Rodolfo
AU - Flores-Cotera, Luis B.
N1 - Funding Information:
Eurotiomycetes were represented by the well-supported Eurotiales (BS = 100 %). Five among seven nodes within the clade, were supported by bootstrap (BS support ≥ 70 %). The endophyte SGLMf44 was reconstructed as sister to Penicillium, and SGLAf37 as sister to Aspergillus/Neosartorya both with strong support. Leotiomycetes were represented by Helotiales (BS = 76 %), with six out of 11 nodes strongly supported (BS ≥ 70 %). This clade contained four isolates, two associated with Cadophora (consistent with morphology), and two fungi of uncertain position i.e. SGSGf25 reconstructed as sister to Torrendiella eucalypti and an uncultured Helotiales from agricultural soil (GU055742), whereas SGLMf22 reconstructed as sister to Lambertella, Sclerotinia, and an uncultured ascomycete from savanna soil ( EU489981 ). Pezizomycetes were represented by Pezizales, an unsupported clade, but with individual branches supported in 16 out of 20 nodes (BS ≥ 70 %). The group contained five isolates clustered with Geopyxis (1), Gyromitra (1), Trichophaea (2) and one isolate associated with an uncultured Pezizomycotina from Lithocarpus densiflorus ( DQ273473 ) and different members of Sarcosomataceae. Our isolates within this group clustered with a number of unknown endolichenic fungi (e.g. HM123309).
PY - 2011/2
Y1 - 2011/2
N2 - We studied the endophytic mycoflora associated with Taxus globosa, the Mexican yew. The study localities; Las Avispas (LA), San Gaspar (SG), and La Mina (LM) were three segments of cloud forest within the range of Sierra Gorda Biosphere Reserve, México. Overall, 245 endophytes were isolated and 105 representative Ascomycota (morphotaxons) were chosen for phylogenetic and genotypic characterization. Maximum likelihood analyses of large subunit of ribosomal RNA (LSU) rDNA showed well-supported clades of Dothideomycetes, Eurotiomycetes, Leotiomycetes, Pezizomycetes, and Sordariomycetes. Analyses of ITS rDNA groups showed 57 genotypes (95% sequence similarity), in general consistent with the phylogenetically delimitated taxa based on LSU rDNA sequences. The endophyte diversity measured by Fisher's α, Shanonn, and Simpson indices was ca. three-fold and ca. two-fold greater in LM than in LA and SG respectively. A screening for paclitaxel using a competitive inhibition enzyme immunoassay showed 16 positive isolates producing between 65 and 250ngl-1. The isolates included Acremonium, Botryosphaeria, Fusarium, Gyromitra, Nigrospora, Penicillium, three novel Pleosporales, and Xylaria.
AB - We studied the endophytic mycoflora associated with Taxus globosa, the Mexican yew. The study localities; Las Avispas (LA), San Gaspar (SG), and La Mina (LM) were three segments of cloud forest within the range of Sierra Gorda Biosphere Reserve, México. Overall, 245 endophytes were isolated and 105 representative Ascomycota (morphotaxons) were chosen for phylogenetic and genotypic characterization. Maximum likelihood analyses of large subunit of ribosomal RNA (LSU) rDNA showed well-supported clades of Dothideomycetes, Eurotiomycetes, Leotiomycetes, Pezizomycetes, and Sordariomycetes. Analyses of ITS rDNA groups showed 57 genotypes (95% sequence similarity), in general consistent with the phylogenetically delimitated taxa based on LSU rDNA sequences. The endophyte diversity measured by Fisher's α, Shanonn, and Simpson indices was ca. three-fold and ca. two-fold greater in LM than in LA and SG respectively. A screening for paclitaxel using a competitive inhibition enzyme immunoassay showed 16 positive isolates producing between 65 and 250ngl-1. The isolates included Acremonium, Botryosphaeria, Fusarium, Gyromitra, Nigrospora, Penicillium, three novel Pleosporales, and Xylaria.
KW - Ascomycota
KW - Diversity
KW - ITS rDNA
KW - Mexican yew
KW - Species richness
UR - http://www.scopus.com/inward/record.url?scp=79551684923&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2010.11.004
DO - 10.1016/j.funbio.2010.11.004
M3 - Artículo
C2 - 21315312
SN - 1878-6146
VL - 115
SP - 143
EP - 156
JO - Fungal Biology
JF - Fungal Biology
IS - 2
ER -