TY - JOUR
T1 - Molecular Identification and Novel Mitochondrial COI Gene Haplotypes of Nesting Kemp’s Ridley Turtles (Lepidochelys kempii) in Rancho Nuevo Sanctuary, Mexico
AU - Camacho‐sánchez, Fátima Yedith
AU - Narváez‐zapata, José Alberto
AU - Acosta‐sánchez, Héctor Hugo
AU - López‐hernández, Martha
AU - Luzariaga‐neira, Agusto
AU - Rodríguez‐gonzález, Hervey
AU - Aguirre, A. Alonso
AU - Reyes‐lópez, Miguel Angel
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5
Y1 - 2022/5
N2 - One hundred new COI sequences of nesting female Kemp’s ridley turtles (Lepidochelys kempii) were obtained in the Rancho Nuevo Sanctuary (RNS). The COI sequences were analyzed and contrasted with others retrieved from BOLD and GenBank with the aim of investigating genetic variability, genetic divergence, and haplotypes of the nesting female population at RNS. Four new COI haplotypes for Kemp’s ridley were described; two are redundant with (LK‐RN01) 97 and (LK‐ COI‐01) 17 specimens belonging to the RNS and other localities, respectively. Nucleotide (0.00052) and haplotype (0.303) diversity showed low and conserved COI values The fixation index (FST) between these main redundant haplotypes showed a high degree of differentiation with ~1. Genetic divergence demonstrated clearly two different Kemp’s ridley nesting populations, one from RNS and a second outside Mexico. Phylogenetic COI analysis was useful to differentiate these redundant (LK‐COI‐01 and RNS LK‐RN01) haplotypes and, therefore, these different Kemp’s ridley populations. In addition, phylogenetic COI analysis clearly separates Kemp’s ridley turtles from other sea turtle species, supporting its use as a barcode marker.
AB - One hundred new COI sequences of nesting female Kemp’s ridley turtles (Lepidochelys kempii) were obtained in the Rancho Nuevo Sanctuary (RNS). The COI sequences were analyzed and contrasted with others retrieved from BOLD and GenBank with the aim of investigating genetic variability, genetic divergence, and haplotypes of the nesting female population at RNS. Four new COI haplotypes for Kemp’s ridley were described; two are redundant with (LK‐RN01) 97 and (LK‐ COI‐01) 17 specimens belonging to the RNS and other localities, respectively. Nucleotide (0.00052) and haplotype (0.303) diversity showed low and conserved COI values The fixation index (FST) between these main redundant haplotypes showed a high degree of differentiation with ~1. Genetic divergence demonstrated clearly two different Kemp’s ridley nesting populations, one from RNS and a second outside Mexico. Phylogenetic COI analysis was useful to differentiate these redundant (LK‐COI‐01 and RNS LK‐RN01) haplotypes and, therefore, these different Kemp’s ridley populations. In addition, phylogenetic COI analysis clearly separates Kemp’s ridley turtles from other sea turtle species, supporting its use as a barcode marker.
KW - COI gene
KW - Kemp’s ridley turtle
KW - Lepidochelys kempii
KW - Mexico
KW - Rancho Nuevo Sanctuary
KW - barcode analysis
KW - endangered species
UR - http://www.scopus.com/inward/record.url?scp=85130686247&partnerID=8YFLogxK
U2 - 10.3390/d14050390
DO - 10.3390/d14050390
M3 - Artículo
AN - SCOPUS:85130686247
SN - 1424-2818
VL - 14
JO - Diversity
JF - Diversity
IS - 5
M1 - 390
ER -