TY - JOUR
T1 - Molecular characterization of human calicivirus associated with acute diarrheal disease in Mexican children
AU - Gámez-Santiago, Fabin
AU - Ribas-Aparicio, Rosa María
AU - García-Lozano, Herlinda
N1 - Funding Information:
We thank the National Network of Public Health Laboratories in Mexico for supplying the biological samples analyzed in this study, the Laboratory of Pathogens Genome-InDRE for technical assistance in the sequencing of RT-PCR products, and Celia Alpuche-Aranda for the revision of and comments on the manuscript. We are grateful for financially supported by Consejo Nacional de Ciencia y Tecnología (CONACyT) Grant number 113740 (to HG-L) and CENAPRECE/DGE-InDRE. HL-G and RMR-A are grateful for support for this work from InDRE-SSA, and SIP-IPN-México grants number 20101151 and 20113197. RMR-A is a recipient of COFAA-IPN and EDD-IPN fellowships. FG-S thanks CONACyT-México and PIFI-IPN-México for graduate studies fellowships.
PY - 2012
Y1 - 2012
N2 - Background. Human caliciviruses (HuCV) are emerging enteric pathogens that are a common cause of diarrhea in humans worldwide. Due to the paucity of information on the molecular characterization of HuCV circulating in Mexico, the aim of this work was to investigate the diversity and molecular epidemiology of the HuCV infection associated with acute diarrheal disease in Mexican children aged up to 5 years. Results. Of the 131/414 (32%) HuCV positive-specimens analyzed, 128 were identified as Norovirus (NoV) and three as Sapovirus (SaV). Of the NoV positive specimens, 118/128 (92%) were NoV GII and 10/128(8%) were untypeable by RT-PCR in both polymerase and capsid genes, whereas one SaV isolate was further confirmed by sequencing as GI.2. Phylogenetic analysis based on polymerase partial gene sequences from 89/131 (68%) HuCV isolates showed that 86/89 (97%) belong to NoV GII.4 with three main variant clusters of this genotype, 2/89 (2%) to NoV GII.2, and 1/89 (1%) to SaV GI.2. Furthermore, partial sequencing of the capsid gene VP1 of 63/131 (48%) strains indicated that 61/63 (97%) correlated with NoV GII.4, whereas only 2/63 (3%) clustered to NoV GII.2. HuCV infections were detected throughout the year, and the highest number of cases positive for NoV was found in children between 7 and 18 months of age (60%). Conclusions. This study highlights the usefulness of analyzing both polymerase and capsid genes for molecular characterization of HuCV and demonstrates the relatedness and predominance of NoV GII.4 with acute diarrheal disease in young Mexican children, thus contributing to better understanding of the molecular epidemiology of this disease.
AB - Background. Human caliciviruses (HuCV) are emerging enteric pathogens that are a common cause of diarrhea in humans worldwide. Due to the paucity of information on the molecular characterization of HuCV circulating in Mexico, the aim of this work was to investigate the diversity and molecular epidemiology of the HuCV infection associated with acute diarrheal disease in Mexican children aged up to 5 years. Results. Of the 131/414 (32%) HuCV positive-specimens analyzed, 128 were identified as Norovirus (NoV) and three as Sapovirus (SaV). Of the NoV positive specimens, 118/128 (92%) were NoV GII and 10/128(8%) were untypeable by RT-PCR in both polymerase and capsid genes, whereas one SaV isolate was further confirmed by sequencing as GI.2. Phylogenetic analysis based on polymerase partial gene sequences from 89/131 (68%) HuCV isolates showed that 86/89 (97%) belong to NoV GII.4 with three main variant clusters of this genotype, 2/89 (2%) to NoV GII.2, and 1/89 (1%) to SaV GI.2. Furthermore, partial sequencing of the capsid gene VP1 of 63/131 (48%) strains indicated that 61/63 (97%) correlated with NoV GII.4, whereas only 2/63 (3%) clustered to NoV GII.2. HuCV infections were detected throughout the year, and the highest number of cases positive for NoV was found in children between 7 and 18 months of age (60%). Conclusions. This study highlights the usefulness of analyzing both polymerase and capsid genes for molecular characterization of HuCV and demonstrates the relatedness and predominance of NoV GII.4 with acute diarrheal disease in young Mexican children, thus contributing to better understanding of the molecular epidemiology of this disease.
KW - Capsid gene
KW - Gastroenteritis
KW - Molecular genotyping
KW - Norovirus
KW - Phylogeny
KW - RdRp gene
KW - Sapovirus
UR - http://www.scopus.com/inward/record.url?scp=84860242460&partnerID=8YFLogxK
U2 - 10.1186/1743-422X-9-54
DO - 10.1186/1743-422X-9-54
M3 - Artículo
C2 - 22361160
SN - 1743-422X
VL - 9
JO - Virology Journal
JF - Virology Journal
M1 - 54
ER -