TY - JOUR
T1 - Molecular Assessment, Drug-Resistant Profile, and Spacer Oligonucleotide Typing (Spoligotyping) of Mycobacterium tuberculosis Strains From Tamaulipas, México
AU - Bocanegra-García, Virgilio
AU - Garza-González, Elvira
AU - Cruz-Pulido, Wendy Lizeth
AU - Guevara-Molina, Yahaira Lizeth
AU - Cantú-Ramírez, Rubén
AU - González, Gloria M.
AU - Rivera, Gildardo
AU - Palma-Nicolas, José P.
PY - 2014/3
Y1 - 2014/3
N2 - Background: Tuberculosis remains a serious global health problem involving one-third of the world population. A wide diversity of Mycobacterium tuberculosis strains cause about 1.5 million deaths/year worldwide, but in developing countries, the genetic diversity of M. tuberculosis strains remains largely unknown. We conducted a first insight into the population diversity of M. tuberculosis strains from Tamaulipas, Mexico. Methods: Seventy-two M. tuberculosis strains were identified and genetic diversity determined by spoligotyping. Drug sensibility testing and punctual mutations in inhA, ahpC, rpoB, and katG genes were assessed. Results: Spoligotyping analysis showed a higher prevalence of LAM9 > T1 > Haarlem3 subfamilies among 53 spoligotype patterns. Unexpectedly, five Beijing strains conforming four unique spoligopatterns were recovered. The more frequently isolated strains (LAM9 and T1), but none of the Beijing strains, were found resistant to INH or RIF. Also, no drug resistance was found among Haarlem3 isolates. The katG315 gene mutation was found in 83% of INH-resistant strains, whereas rpoB526 were associated in only 43% of RIF M. tuberculosis drug-resistant strains. Conclusions: This and other studies report a high rate of orphan spoligotypes, which highlights the need for genotyping implementation as a routine technique for better understanding of M. tuberculosis strains in developing countries such as Mexico.
AB - Background: Tuberculosis remains a serious global health problem involving one-third of the world population. A wide diversity of Mycobacterium tuberculosis strains cause about 1.5 million deaths/year worldwide, but in developing countries, the genetic diversity of M. tuberculosis strains remains largely unknown. We conducted a first insight into the population diversity of M. tuberculosis strains from Tamaulipas, Mexico. Methods: Seventy-two M. tuberculosis strains were identified and genetic diversity determined by spoligotyping. Drug sensibility testing and punctual mutations in inhA, ahpC, rpoB, and katG genes were assessed. Results: Spoligotyping analysis showed a higher prevalence of LAM9 > T1 > Haarlem3 subfamilies among 53 spoligotype patterns. Unexpectedly, five Beijing strains conforming four unique spoligopatterns were recovered. The more frequently isolated strains (LAM9 and T1), but none of the Beijing strains, were found resistant to INH or RIF. Also, no drug resistance was found among Haarlem3 isolates. The katG315 gene mutation was found in 83% of INH-resistant strains, whereas rpoB526 were associated in only 43% of RIF M. tuberculosis drug-resistant strains. Conclusions: This and other studies report a high rate of orphan spoligotypes, which highlights the need for genotyping implementation as a routine technique for better understanding of M. tuberculosis strains in developing countries such as Mexico.
KW - Beijing
KW - Spoligotyping
KW - Tuberculosis
KW - katG
KW - rpoB
UR - http://www.scopus.com/inward/record.url?scp=84896129139&partnerID=8YFLogxK
U2 - 10.1002/jcla.21650
DO - 10.1002/jcla.21650
M3 - Artículo
C2 - 24395541
SN - 0887-8013
VL - 28
SP - 97
EP - 103
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
IS - 2
ER -