TY - JOUR
T1 - MiR-485 acts as a tumor suppressor by inhibiting cell growth and migration in breast carcinoma T47D cells
AU - Anaya-Ruiz, Maricruz
AU - Bandala, Cindy
AU - Pérez-Santos, José Luis Martín
PY - 2013
Y1 - 2013
N2 - MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells.Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR- 485 might play an important role in breast cancer by suppressing cell proliferation and migration.
AB - MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells.Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR- 485 might play an important role in breast cancer by suppressing cell proliferation and migration.
KW - Breast cancer
KW - MicroRNA
KW - Migration
KW - Tumor suppressor
UR - http://www.scopus.com/inward/record.url?scp=84882638790&partnerID=8YFLogxK
U2 - 10.7314/APJCP.2013.14.6.3757
DO - 10.7314/APJCP.2013.14.6.3757
M3 - Artículo
AN - SCOPUS:84882638790
SN - 1513-7368
VL - 14
SP - 3757
EP - 3760
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
IS - 6
ER -