TY - JOUR
T1 - Maintenance of Acanthamoeba culbertsoni by cryopreservation
AU - Alejandre-Agullar, Ricardo
AU - Calvo-Méndez, Ma Lourdes
AU - Nogueda-Torres, Benjamin
AU - De La Jara-Alcocer, Fernando
PY - 1998
Y1 - 1998
N2 - In order to determine the viability of trophozoites of Acanthamoeba culbertsoni under cryopreservation conditions, cultures in serum-casein- glucose-yeast extract medium were subject to 5%, 7.5%, and 10% concentrations of dimethylsulfoxide (DMSO). With the methodology followed, the percentages of recovery varied between 75.6% and 86.6% with DMSO at 10%, between 54.5% and 73.5% with DMSO at 7.5%, and between 43.6% and 68.5% with DMSO at 5%. The amebae were kept in liquid nitrogen for 30-210 days. The highest viability of trophozoites was founded when DMSO was used at a final concentration of 10% and an equilibrium temperature of 4 C. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.
AB - In order to determine the viability of trophozoites of Acanthamoeba culbertsoni under cryopreservation conditions, cultures in serum-casein- glucose-yeast extract medium were subject to 5%, 7.5%, and 10% concentrations of dimethylsulfoxide (DMSO). With the methodology followed, the percentages of recovery varied between 75.6% and 86.6% with DMSO at 10%, between 54.5% and 73.5% with DMSO at 7.5%, and between 43.6% and 68.5% with DMSO at 5%. The amebae were kept in liquid nitrogen for 30-210 days. The highest viability of trophozoites was founded when DMSO was used at a final concentration of 10% and an equilibrium temperature of 4 C. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.
UR - http://www.scopus.com/inward/record.url?scp=0032438314&partnerID=8YFLogxK
U2 - 10.2307/3284685
DO - 10.2307/3284685
M3 - Artículo
SN - 0022-3395
VL - 84
SP - 1261
EP - 1263
JO - Journal of Parasitology
JF - Journal of Parasitology
IS - 6
ER -