TY - JOUR
T1 - Macrophage activation by ursolic and oleanolic acids during mycobacterial infection
AU - López-García, Sonia
AU - Castañeda-Sanchez, Jorge Ismael
AU - Jiménez-Arellanes, Adelina
AU - Domínguez-López, Lilia
AU - Castro-Mussot, Maria Eugenia
AU - Hernández-Sanchéz, Javier
AU - Luna-Herrera, Julieta
N1 - Publisher Copyright:
© 2015 by the authors.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Oleanolic (OA) and ursolic acids (UA) are triterpenes that are abundant in vegetables, fruits and medicinal plants. They have been described as active moieties in medicinal plants used for the treatment of tuberculosis. In this study, we analyzed the effects of these triterpenes on macrophages infected in vitro with Mycobacterium tuberculosis (MTB). We evaluated production of nitric oxide (NO), reactive oxygen species (ROS), and cytokines (TNF-α and TGF-β) as well as expression of cell membrane receptors (TGR5 and CD36) in MTB-infected macrophages following treatment with OA and UA. Triterpenes caused reduced MTB growth in macrophages, stimulated production of NO and ROS in the early phase, stimulated TNF-α, suppressed TGF-β and caused over-expression of CD36and TGR5 receptors. Thus, our data suggest immunomodulatory properties of OA and UA on MTB infected macrophages. In conclusion, antimycobacterial effects induced by these triterpenes may be attributable to the conversion of macrophages from stage M2 (alternatively activated) to M1 (classically activated).
AB - Oleanolic (OA) and ursolic acids (UA) are triterpenes that are abundant in vegetables, fruits and medicinal plants. They have been described as active moieties in medicinal plants used for the treatment of tuberculosis. In this study, we analyzed the effects of these triterpenes on macrophages infected in vitro with Mycobacterium tuberculosis (MTB). We evaluated production of nitric oxide (NO), reactive oxygen species (ROS), and cytokines (TNF-α and TGF-β) as well as expression of cell membrane receptors (TGR5 and CD36) in MTB-infected macrophages following treatment with OA and UA. Triterpenes caused reduced MTB growth in macrophages, stimulated production of NO and ROS in the early phase, stimulated TNF-α, suppressed TGF-β and caused over-expression of CD36and TGR5 receptors. Thus, our data suggest immunomodulatory properties of OA and UA on MTB infected macrophages. In conclusion, antimycobacterial effects induced by these triterpenes may be attributable to the conversion of macrophages from stage M2 (alternatively activated) to M1 (classically activated).
KW - CD36
KW - Macrophages
KW - Nitric oxide (NO)
KW - Oleanolic acid
KW - Reactive oxygen species (ROS)
KW - TGR5
KW - Transforming growth factor (TGF-β)
KW - Triterpenes
KW - Tuberculosis
KW - Tumor necrosis factor (TNF-α)
KW - Ursolic acid
UR - http://www.scopus.com/inward/record.url?scp=84941254050&partnerID=8YFLogxK
U2 - 10.3390/molecules200814348
DO - 10.3390/molecules200814348
M3 - Artículo
C2 - 26287131
SN - 1420-3049
VL - 20
SP - 14348
EP - 14364
JO - Molecules
JF - Molecules
IS - 8
ER -