TY - JOUR
T1 - Lymphocytes and B-cell abnormalities in patients with common variable immunodeficiency (CVID)
AU - Berrón-Ruiz, L.
AU - López-Herrera, G.
AU - Vargas-Hernández, A.
AU - Mogica-Martínez, D.
AU - García-Latorre, E.
AU - Blancas-Galicia, L.
AU - Espinosa-Rosales, F. J.
AU - Santos-Argumedo, L.
N1 - Funding Information:
This work was partially supported by the Instituto de Ciencia y Tecnología del Distrito Federal (grant ICYTDF/326/2009 ), the Consejo Nacional de Ciencia y Tecnología (CONACYT, México) (grant #’s 56836 and 58945 ); and the Fundación Mexicana para los Niñas y Niños con Inmunodeficiencias Primarias A.C.
Funding Information:
Gabriela López-Herrera, Francisco Espinosa-Rosales, Ethel García-Latorre and Leopoldo Santos-Argumedo are SNI fellows. Ethel García-Latorre is a fellow of COFAA, EDI, and IPN. Laura Berrón-Ruiz was the recipient of a doctoral (176353) and postdoctoral (CVU 44813) scholarship from CONACYT, México.
PY - 2014/1
Y1 - 2014/1
N2 - Background and aims: Common variable immunodeficiency (CVID) is a primary antibody deficiency characterised by decreased antibody production and low or normal B-cell numbers. To elucidate the clinical and immunological heterogeneity of CVID, we studied 16 patients diagnosed with CVID. Methods: We analysed B, T and NK cell populations. We also assessed CD27 expression to define B-cell subsets and examined the expression of molecules important in B-cell proliferation and differentiation, such as the transmembrane activator and CALM interactor (TACI), inducible costimulator (ICOS), CD154 and CD40. Results: We observed reduced B and T-cell numbers in CVID patients; this reduction was more pronounced in adults. While one group of patients (group I) showed a significant reduction in CD27+ memory B-cells, another group (group II) of patients exhibited numbers of CD27+ memory B-cells similar to the healthy donor. The frequency of B-cells and T-cells expressing CD40 and ICOS, respectively, was significantly lower in all CVID patients compared with healthy donors. Finally, a correlation between the frequency of CD27+ memory B-cells and clinical features was observed in CVID patients. Conclusion: These results suggest that in some patients, the combined defects in both T and B-cells may account for CVID. Additionally, patients in group I exhibited an increased frequency of pneumonia and chronic diarrhoea.
AB - Background and aims: Common variable immunodeficiency (CVID) is a primary antibody deficiency characterised by decreased antibody production and low or normal B-cell numbers. To elucidate the clinical and immunological heterogeneity of CVID, we studied 16 patients diagnosed with CVID. Methods: We analysed B, T and NK cell populations. We also assessed CD27 expression to define B-cell subsets and examined the expression of molecules important in B-cell proliferation and differentiation, such as the transmembrane activator and CALM interactor (TACI), inducible costimulator (ICOS), CD154 and CD40. Results: We observed reduced B and T-cell numbers in CVID patients; this reduction was more pronounced in adults. While one group of patients (group I) showed a significant reduction in CD27+ memory B-cells, another group (group II) of patients exhibited numbers of CD27+ memory B-cells similar to the healthy donor. The frequency of B-cells and T-cells expressing CD40 and ICOS, respectively, was significantly lower in all CVID patients compared with healthy donors. Finally, a correlation between the frequency of CD27+ memory B-cells and clinical features was observed in CVID patients. Conclusion: These results suggest that in some patients, the combined defects in both T and B-cells may account for CVID. Additionally, patients in group I exhibited an increased frequency of pneumonia and chronic diarrhoea.
KW - CD154
KW - CD40
KW - CVID
KW - ICOS
KW - Memory B-cells
KW - TACI
UR - http://www.scopus.com/inward/record.url?scp=84893823578&partnerID=8YFLogxK
U2 - 10.1016/j.aller.2012.07.016
DO - 10.1016/j.aller.2012.07.016
M3 - Artículo
C2 - 23305827
SN - 0301-0546
VL - 42
SP - 35
EP - 43
JO - Allergologia et Immunopathologia
JF - Allergologia et Immunopathologia
IS - 1
ER -