TY - JOUR
T1 - lncRNAs dysregulation in monocytes from primary antiphospholipid syndrome patients
T2 - a bioinformatic and an experimental proof-of-concept approach
AU - Guzmán-Martín, Carlos A.
AU - Juárez-Vicuña, Yaneli
AU - Domínguez-López, Aarón
AU - González-Ramírez, Javier
AU - Amezcua-Guerra, Luis M.
AU - Martínez-Martínez, Laura A.
AU - Sánchez-Muñoz, Fausto
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/1
Y1 - 2023/1
N2 - Background: Antiphospholipid syndrome (APS) is the main cause of acquired thrombophilia where peripheral circulating cells such as monocytes have a key role. Currently, several studies have linked long non-coding RNAs (lncRNAs) in different inflammatory and autoimmune processes, including lupus. However, the role of lncRNAs in antiphospholipid syndrome is unknown, therefore, we aimed to select and measure expression levels of three lncRNAs based on its abundance in monocytes from APS patients. Methods: Selection of lncRNAs candidates were carried out based on its abundance in monocytes and their relationship with Perez-Sanchez miRNA signature by using miRNet 2.0 bioinformatic tool, then lncRNAs expression levels was measured in monocytes by RT-qPCR. Results: This is the first study to report that lncRNAs: FGD5-AS1, OIP5-AS1 and GAS5 are promising candidates for play a role on APS monocytes and they are expressed differently between patients and controls. Conclusions: OIP5-AS1, FGD5-AS1 and GAS5 are downregulated on monocytes from APS patients.
AB - Background: Antiphospholipid syndrome (APS) is the main cause of acquired thrombophilia where peripheral circulating cells such as monocytes have a key role. Currently, several studies have linked long non-coding RNAs (lncRNAs) in different inflammatory and autoimmune processes, including lupus. However, the role of lncRNAs in antiphospholipid syndrome is unknown, therefore, we aimed to select and measure expression levels of three lncRNAs based on its abundance in monocytes from APS patients. Methods: Selection of lncRNAs candidates were carried out based on its abundance in monocytes and their relationship with Perez-Sanchez miRNA signature by using miRNet 2.0 bioinformatic tool, then lncRNAs expression levels was measured in monocytes by RT-qPCR. Results: This is the first study to report that lncRNAs: FGD5-AS1, OIP5-AS1 and GAS5 are promising candidates for play a role on APS monocytes and they are expressed differently between patients and controls. Conclusions: OIP5-AS1, FGD5-AS1 and GAS5 are downregulated on monocytes from APS patients.
KW - Antiphospholipid syndrome
KW - FGD5-AS1
KW - GAS5
KW - Long noncoding RNAs
KW - Monocytes
KW - OIP5-AS1
UR - http://www.scopus.com/inward/record.url?scp=85141689644&partnerID=8YFLogxK
U2 - 10.1007/s11033-022-08080-y
DO - 10.1007/s11033-022-08080-y
M3 - Artículo
C2 - 36367661
AN - SCOPUS:85141689644
SN - 0301-4851
VL - 50
SP - 937
EP - 941
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 1
ER -