TY - JOUR
T1 - Lipopopeptidephosphoglycan from Entamoeba histolytica activates human macrophages and dendritic cells and reaches their late endosomes
AU - Vivanco-Cid, H.
AU - Alpuche-Aranda, C.
AU - Wong-Baeza, I.
AU - Rocha-Ramírez, L. M.
AU - Rios-Sarabia, N.
AU - Estrada-Garcia, I.
AU - Villasis-Keever, M. A.
AU - Lopez-Macias, C.
AU - Isibasi, A.
PY - 2007/9
Y1 - 2007/9
N2 - Lipopopeptidephosphoglycan (LPPG) is a complex macromolecule from the surface of Entamoeba histolytica trophozoites. We analysed the interaction between LPPG and human macrophages and dendritic cells (DCs) and found that LPPG is internalized by these cells and activates them. The internalization process involves intracellular traffic from the cell membrane to late endosomes, as shown by co-localization of LPPG with late endosomes marked with FITC-dextran and LAMP-1. LPPG-activated DCs have increased expression of co-stimulatory molecules CD80, CD86 and CD40 and produce pro-inflammatory cytokines TNF-α, IL-8 and IL-12. Taken together, these results show that LPPG activates antigen-presenting cells and reaches intracellular compartments that are involved in antigen presentation.
AB - Lipopopeptidephosphoglycan (LPPG) is a complex macromolecule from the surface of Entamoeba histolytica trophozoites. We analysed the interaction between LPPG and human macrophages and dendritic cells (DCs) and found that LPPG is internalized by these cells and activates them. The internalization process involves intracellular traffic from the cell membrane to late endosomes, as shown by co-localization of LPPG with late endosomes marked with FITC-dextran and LAMP-1. LPPG-activated DCs have increased expression of co-stimulatory molecules CD80, CD86 and CD40 and produce pro-inflammatory cytokines TNF-α, IL-8 and IL-12. Taken together, these results show that LPPG activates antigen-presenting cells and reaches intracellular compartments that are involved in antigen presentation.
KW - Dendritic cells
KW - Entamoeba histolytica
KW - Lipopopeptidephosphoglycan
KW - Macrophages
UR - http://www.scopus.com/inward/record.url?scp=34548189709&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3024.2007.00963.x
DO - 10.1111/j.1365-3024.2007.00963.x
M3 - Artículo
C2 - 17727570
SN - 0141-9838
VL - 29
SP - 467
EP - 474
JO - Parasite Immunology
JF - Parasite Immunology
IS - 9
ER -