TY - JOUR
T1 - Ligninolytic activity patterns of pleurotus ostreatus obtained by submerged fermentation in presence of 2,6-dimethoxyphenol and remazol brilliant blue R dye
AU - Grandes-Blanco, Amado I.
AU - Díaz-Godínez, Gerardo
AU - Téllez-Téllez, Maura
AU - Delgado-Macuil, Raúl J.
AU - Rojas-López, Marlon
AU - Bibbins-Martínez, Martha D.
N1 - Funding Information:
We thank the Mexican Council of Science and Technology (CONACYT) for supporting this research (project no. 134348).
PY - 2013/4/15
Y1 - 2013/4/15
N2 - The degradation of 2,6-dimethoxyphenol (DMP) and decolorization of Remazol brilliant blue R dye (RBB), added to culture media of Pleurotus ostreatus developed in submerged fermentation, and the laccase, manganese peroxidase and veratryl alcohol oxidase activities produced in these systems were evaluated. Both compounds were removed from the culture medium mainly by enzymatic action. These compounds decreased the specific growth rate and the effect on the maximal biomass values was not important. The enzymatic activities were increased by DMP and/or RBB; however, the DMP showed a higher inducer effect on all enzymes than RBB. On the other hand, the RBB showed a larger inducer effect on manganese peroxidase activity than on the laccases and veratryl alcohol oxidase activities. These results show that DMP was a better inducer of ligninolytic enzymes than dye, and the process of dye decolorization and degradation of DMP requires the action of all enzymes of the ligninolytic complex.
AB - The degradation of 2,6-dimethoxyphenol (DMP) and decolorization of Remazol brilliant blue R dye (RBB), added to culture media of Pleurotus ostreatus developed in submerged fermentation, and the laccase, manganese peroxidase and veratryl alcohol oxidase activities produced in these systems were evaluated. Both compounds were removed from the culture medium mainly by enzymatic action. These compounds decreased the specific growth rate and the effect on the maximal biomass values was not important. The enzymatic activities were increased by DMP and/or RBB; however, the DMP showed a higher inducer effect on all enzymes than RBB. On the other hand, the RBB showed a larger inducer effect on manganese peroxidase activity than on the laccases and veratryl alcohol oxidase activities. These results show that DMP was a better inducer of ligninolytic enzymes than dye, and the process of dye decolorization and degradation of DMP requires the action of all enzymes of the ligninolytic complex.
KW - Pleurotus ostreatus
KW - biomass
KW - dye
KW - enzyme activity
KW - filamentous fungi
KW - submerged culture
UR - http://www.scopus.com/inward/record.url?scp=84876354320&partnerID=8YFLogxK
U2 - 10.1080/10826068.2012.746233
DO - 10.1080/10826068.2012.746233
M3 - Artículo
C2 - 23581782
SN - 1082-6068
VL - 43
SP - 468
EP - 480
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
IS - 5
ER -