TY - JOUR
T1 - Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center
AU - Cortés-Ortíz, Iliana Alejandra
AU - Juárez-Gómez, Julio Cesar
AU - Cu-Quijano, Concepción
AU - Flores-Paz, Rocio
AU - Durán-Manuel, Emilio Mariano
AU - Cruz-Cruz, Clemente
AU - Gutiérrez-Muñoz, Victor Hugo
AU - Sosa-Hernández, Oscar
AU - Escobar-Escamilla, Noé
AU - Bravata-Alcántara, Juan Carlos
AU - Delgado-Balbuena, Laura
AU - Ibáñez-Cervantes, Gabriela
AU - Bello-López, Juan Manuel
N1 - Publisher Copyright:
Copyright © 2021 Cortés-Ortíz et al.
PY - 2021/5
Y1 - 2021/5
N2 - Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel.
AB - Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel.
KW - BlaNDM-1
KW - Class 1 integron
KW - Hospital outbreak
KW - Klebsiella pneumoniae
UR - http://www.scopus.com/inward/record.url?scp=85107986584&partnerID=8YFLogxK
U2 - 10.3855/JIDC.12996
DO - 10.3855/JIDC.12996
M3 - Artículo
C2 - 34106889
AN - SCOPUS:85107986584
SN - 2036-6590
VL - 15
SP - 657
EP - 664
JO - Journal of Infection in Developing Countries
JF - Journal of Infection in Developing Countries
IS - 5
ER -