TY - JOUR
T1 - Kinetic study of δ-Ala induced porphyrins in mice using photoacoustic and fluorescence spectroscopies
AU - Stolik, Suren
AU - Tomás, Sergio A.
AU - Ramón-Gallegos, Eva
AU - Sánchez, Feliciano
N1 - Funding Information:
This work was partially supported by CONACyT through project No. 33126-E. S.S. is grateful to CONACyT for a grant received via this project.
PY - 2002/11
Y1 - 2002/11
N2 - The production of δ-aminolevulinic acid (ALA)-induced porphyrins in mice skin and blood was studied by photoacoustic and fluorescence spectroscopies. Mice were intraperitoneally administered with 30 mg/kg of ALA. The abdominal skin was subsequently excised at specific times within an 8-h interval and its absorption spectrum obtained by photoacoustics. The highest porphyrins concentration in skin, determined from the optical absorption of the Soret band at 410 nm, was found to occur nearly 2 h after ALA administration, but a first peak was also observed at approximately 15 min. Our hypothesis that the first peak represents the porphyrins content in blood vessels within the skin, whereas the second peak corresponds to porphyrins production in skin tissue, was confirmed by analysing the evolution of protoporphyrin IX content in plasma extracted intracardiacally. By finally applying phase resolved photoacoustic spectroscopy, we were able to evaluate the mean depth at which porphyrins are generated.
AB - The production of δ-aminolevulinic acid (ALA)-induced porphyrins in mice skin and blood was studied by photoacoustic and fluorescence spectroscopies. Mice were intraperitoneally administered with 30 mg/kg of ALA. The abdominal skin was subsequently excised at specific times within an 8-h interval and its absorption spectrum obtained by photoacoustics. The highest porphyrins concentration in skin, determined from the optical absorption of the Soret band at 410 nm, was found to occur nearly 2 h after ALA administration, but a first peak was also observed at approximately 15 min. Our hypothesis that the first peak represents the porphyrins content in blood vessels within the skin, whereas the second peak corresponds to porphyrins production in skin tissue, was confirmed by analysing the evolution of protoporphyrin IX content in plasma extracted intracardiacally. By finally applying phase resolved photoacoustic spectroscopy, we were able to evaluate the mean depth at which porphyrins are generated.
KW - Aminolevulinic acid
KW - Photoacoustic spectroscopy
KW - Photodynamic therapy
KW - Protoporphyrin IX
UR - http://www.scopus.com/inward/record.url?scp=0036867640&partnerID=8YFLogxK
U2 - 10.1016/S1011-1344(02)00381-0
DO - 10.1016/S1011-1344(02)00381-0
M3 - Artículo
SN - 1011-1344
VL - 68
SP - 117
EP - 122
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
IS - 2-3
ER -