TY - JOUR
T1 - Isolation and expression of cytochrome P450 genes in the antennae and gut of pine beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) following exposure to host monoterpenes
AU - Cano-Ramírez, Claudia
AU - López, María Fernanda
AU - Cesar-Ayala, Ana K.
AU - Pineda-Martínez, Verónica
AU - Sullivan, Brian T.
AU - Zúñiga, Gerardo
N1 - Funding Information:
We are grateful to Jesús I. Morales Jiménez and Gabriel Obregon, for their assistance in collecting insects, and to administrator Jaime Chávez and COPAMEX for providing us access to installations and for his logistic support in San Juanito Chihuahua. We thank Graciela Castro Escarpulli, Javier Victor, Isabel Salazar, Jorge Macías Sámano, and Gloria León Avila for their comments and valuable suggestions regarding the manuscript. The project was funded by the Comisión Nacional Forestal-Consejo Nacional de Ciencia y Tecnología (CONAFOR-CONACYT, 69539 ), Consejo Nacional de Ciencia y Tecnología ( 181337/CB2012 ) and Secretaría de Investigación y Posgrado-IPN ( SIP-20090576 ). This work was part of CCR and MFL Ph Ds. dissertations. They were fellows of Consejo Nacional de Ciencia y Tecnología ( 202060 , 175839 respectively) and Programa Institucional de Formación de Investigadores del Instituto Politécnico Nacional (PIFI-IPN) . Karina Cesar Ayala and Verónica Pineda Martínez were CONAFOR-CONACYT and PIFI-IPN fellows.
PY - 2013/5/10
Y1 - 2013/5/10
N2 - Bark beetles oxidize the defensive monoterpenes of their host trees both to detoxify them and convert them into components of their pheromone system. This oxidation is catalyzed by cytochrome P450 enzymes and occurs in different tissues of the insect, including the gut (i.e., the site where the beetle's pheromones are produced and accumulated) and the antennae (i.e., the olfactory organs used for perception of airborne defensive monoterpenes as well as other host-associated compounds and pheromones). We identified ten new CYP genes in the pine beetle Dendroctonus rhizophagus in either antennae or gut tissue after stimulation with the vapors of major host monoterpenes α-pinene, β-pinene and 3-carene. Five genes belong to the CYP4 family, four to the CYP6 family and one to the CYP9 family. Differential expression of almost all of the CYP genes was observed between sexes, and within these significant differences among time, stimuli, anatomical region, and their interactions were found upon exposure to host monoterpenes. Increased expression of cytochrome P450 genes suggests that they play a role in the detoxification of monoterpenes released by this insect's host trees.
AB - Bark beetles oxidize the defensive monoterpenes of their host trees both to detoxify them and convert them into components of their pheromone system. This oxidation is catalyzed by cytochrome P450 enzymes and occurs in different tissues of the insect, including the gut (i.e., the site where the beetle's pheromones are produced and accumulated) and the antennae (i.e., the olfactory organs used for perception of airborne defensive monoterpenes as well as other host-associated compounds and pheromones). We identified ten new CYP genes in the pine beetle Dendroctonus rhizophagus in either antennae or gut tissue after stimulation with the vapors of major host monoterpenes α-pinene, β-pinene and 3-carene. Five genes belong to the CYP4 family, four to the CYP6 family and one to the CYP9 family. Differential expression of almost all of the CYP genes was observed between sexes, and within these significant differences among time, stimuli, anatomical region, and their interactions were found upon exposure to host monoterpenes. Increased expression of cytochrome P450 genes suggests that they play a role in the detoxification of monoterpenes released by this insect's host trees.
KW - Bark beetles
KW - Cytochrome P450
KW - Detoxification
KW - Gene expression
KW - Scolytines
UR - http://www.scopus.com/inward/record.url?scp=84875807467&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2012.11.059
DO - 10.1016/j.gene.2012.11.059
M3 - Artículo
C2 - 23262344
SN - 0378-1119
VL - 520
SP - 47
EP - 63
JO - Gene
JF - Gene
IS - 1
ER -