TY - JOUR
T1 - Isolation and characterization of potential probiotic bacteria suitable for mollusk larvae cultures
AU - Escamilla-Montes, Ruth
AU - Luna-González, Antonio
AU - Flores-Miranda, Ma del Carmen
AU - Álvarez-Ruiz, Píndaro
AU - Fierro-Coronado, Jesús Arturo
AU - Sánchez-Ortiz, Ana Claudia
AU - Ávila-Leal, José
PY - 2015
Y1 - 2015
N2 - Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and some resistance to antibiotics. The isolates were identified as Bacillus licheniformis (BCR 4-3) and Leuconostoc mesenteroides (BALCR 5-2 and BALOR 2-3). The strains showed basic characteristics to be used as probiotics in mollusk larvae cultures.
AB - Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and some resistance to antibiotics. The isolates were identified as Bacillus licheniformis (BCR 4-3) and Leuconostoc mesenteroides (BALCR 5-2 and BALOR 2-3). The strains showed basic characteristics to be used as probiotics in mollusk larvae cultures.
KW - Bacilli
KW - Lactic acid bacteria
KW - Larvae
KW - Mollusks
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=84929326998&partnerID=8YFLogxK
M3 - Artículo
SN - 0125-6491
VL - 45
SP - 11
EP - 21
JO - Thai Journal of Veterinary Medicine
JF - Thai Journal of Veterinary Medicine
IS - 1
ER -