TY - JOUR
T1 - Inorganic phosphate solubilization by a novel isolated bacterial strain enterobacter sp. Itcb-09 and its application potential as biofertilizer
AU - Mendoza-Arroyo, Gustavo Enrique
AU - Chan-Bacab, Manuel Jesús
AU - Aguila-Ramírez, Ruth Noemi
AU - Ortega-Morales, Benjamín Otto
AU - Solís, René Efraín Canché
AU - Chab-Ruiz, Antonio O.
AU - Cob-Rivera, Katia I.
AU - Dzib-Castillo, Benito
AU - Tun-Che, Rodrigo Enrique
AU - Camacho-Chab, Juan Carlos
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/9
Y1 - 2020/9
N2 - The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.
AB - The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.
KW - Biofertilizers
KW - Enterobacter sp
KW - Extracellular polymeric substances
KW - Phosphate solubilization
KW - Siderophores
UR - http://www.scopus.com/inward/record.url?scp=85093906973&partnerID=8YFLogxK
U2 - 10.3390/agriculture10090383
DO - 10.3390/agriculture10090383
M3 - Artículo
AN - SCOPUS:85093906973
SN - 2077-0472
VL - 10
SP - 1
EP - 15
JO - Agriculture (Switzerland)
JF - Agriculture (Switzerland)
IS - 9
M1 - 383
ER -