TY - JOUR
T1 - Inhibition of dendritic cell apoptosis by Leishmania mexicana amastigotes
AU - Gutiérrez-Kobeh, Laila
AU - De Oyarzabal, Eleané
AU - Argueta, Jesús
AU - Wilkins, Arturo
AU - Salaiza, Norma
AU - Fernández, Edith
AU - López, Oliver
AU - Aguirre, Magdalena
AU - Becker, Ingeborg
N1 - Funding Information:
Acknowledgments Eleané de Oyarzabal was recipient of a scholarship from DGAPA, UNAM project number IN211510. This work was supported by grants IN211510 from DGAPA-UNAM and 79996 from CONACyT.
PY - 2013/4
Y1 - 2013/4
N2 - Macrophages (Mφ) and dendritic cells are the major target cell populations of the obligate intracellular parasite Leishmania. Inhibition of host cell apoptosis is a strategy employed by multiple pathogens to ensure their survival in the infected cell. Leishmania promastigotes have been shown to protect Mφ, neutrophils, and dendritic cells from both natural and induced apoptosis. Nevertheless, the effect of the infection with Leishmania amastigotes in the apoptosis of these cell populations has not been established, which results are very important since amastigotes persist in cells for many days and are responsible for sustaining infection in the host. As shown in this study, apoptosis of monocyte-derived dendritic cells (moDC) induced by treatment with camptothecin was downregulated by infection with L. mexicana amastigotes from 42.48 to 36.92 % as detected by Annexin-V binding to phosphatidylserine. Also, the infection of moDC with L. mexicana amastigotes diminished the fragmentation of DNA as detected by terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling assay, and changes in cell morphology were analyzed by electron microscopy. The observed antiapoptotic effect was found to be associated with an 80 % reduction in the presence of active caspase-3 in infected moDC. The capacity of L. mexicana amastigotes to delay apoptosis induction in the infected moDC may have implications for Leishmania pathogenesis by favoring the invasion of its host and the persistence of the parasite in the infected cells.
AB - Macrophages (Mφ) and dendritic cells are the major target cell populations of the obligate intracellular parasite Leishmania. Inhibition of host cell apoptosis is a strategy employed by multiple pathogens to ensure their survival in the infected cell. Leishmania promastigotes have been shown to protect Mφ, neutrophils, and dendritic cells from both natural and induced apoptosis. Nevertheless, the effect of the infection with Leishmania amastigotes in the apoptosis of these cell populations has not been established, which results are very important since amastigotes persist in cells for many days and are responsible for sustaining infection in the host. As shown in this study, apoptosis of monocyte-derived dendritic cells (moDC) induced by treatment with camptothecin was downregulated by infection with L. mexicana amastigotes from 42.48 to 36.92 % as detected by Annexin-V binding to phosphatidylserine. Also, the infection of moDC with L. mexicana amastigotes diminished the fragmentation of DNA as detected by terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling assay, and changes in cell morphology were analyzed by electron microscopy. The observed antiapoptotic effect was found to be associated with an 80 % reduction in the presence of active caspase-3 in infected moDC. The capacity of L. mexicana amastigotes to delay apoptosis induction in the infected moDC may have implications for Leishmania pathogenesis by favoring the invasion of its host and the persistence of the parasite in the infected cells.
UR - http://www.scopus.com/inward/record.url?scp=84876406300&partnerID=8YFLogxK
U2 - 10.1007/s00436-013-3334-2
DO - 10.1007/s00436-013-3334-2
M3 - Artículo
C2 - 23420408
SN - 0932-0113
VL - 112
SP - 1755
EP - 1762
JO - Parasitology Research
JF - Parasitology Research
IS - 4
ER -