TY - JOUR
T1 - In vivo expression of helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer
AU - Avilés-Jiménez, Francisco
AU - Reyes-Leon, Adriana
AU - Nieto-Patlán, Erik
AU - Hansen, Lori M.
AU - Burgueño, Juan
AU - Ramos, Irma P.
AU - Camorlinga-Ponce, Margarita
AU - Bermúdez, Hector
AU - Blancas, Juan M.
AU - Cabrera, Lourdes
AU - Ribas-Aparicio, Rosa María
AU - Solnick, Jay V.
AU - Torres-López, Javier
N1 - Funding Information:
This research was undertaken with the financial support and volunteers of Earthwatch International and Central Queensland Koala Volunteers; the Koala Study Program, University of Queensland; and the staff of the Queensland Parks and Wildlife Service. In particular, the assistance of Sean FitzGibbon (The University of Queensland), Delma Clifton and Gail Tucker (Central
PY - 2012/2
Y1 - 2012/2
N2 - The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host epithelial cells. Here we used real-time reverse transcription-PCR (RT-PCR) to measure the in vivo expression of genes on the cagPAI and of other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely a result of environmental conditions in the gastric mucosa, though it may in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU.
AB - The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host epithelial cells. Here we used real-time reverse transcription-PCR (RT-PCR) to measure the in vivo expression of genes on the cagPAI and of other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely a result of environmental conditions in the gastric mucosa, though it may in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU.
UR - http://www.scopus.com/inward/record.url?scp=84857173944&partnerID=8YFLogxK
U2 - 10.1128/IAI.05845-11
DO - 10.1128/IAI.05845-11
M3 - Artículo
C2 - 22124657
SN - 0019-9567
VL - 80
SP - 594
EP - 601
JO - Infection and Immunity
JF - Infection and Immunity
IS - 2
ER -