TY - JOUR
T1 - In-vitro secretion of proinflammatory cytokines by human amniochorion carrying hyper-responsive gene polymorphisms of tumour necrosis factor-α and interleukin-1β
AU - Hernandez-Guerrero, C.
AU - Monzon-Bordonaba, F.
AU - Jimenez-Zamudio, L.
AU - Ahued-Ahued, R.
AU - Arechavaleta-Velasco, F.
AU - Strauss, J. F.
AU - Vadillo-Ortega, Felipe
N1 - Funding Information:
This study was supported by CONACyT grants 34743-M and 26177-M, Fellowship 91993 from CONACYT/C.Q.B., Biomedicine Ph.D. Program (to C.H.-G.) and Fellowship COFAA and EDD, IPN (to L.J.-Z.).
PY - 2003/10/1
Y1 - 2003/10/1
N2 - The identification of polymorphisms in genes encoding proinflammatory cytokines that affect transcription or the secretion rate has opened new ways to understand the variation in responses to infection during pregnancy. In this study, human amniochorion carrying hyper-responsive alleles of tumour necrosis factor-α (TNF-α: TNF*2 at -308) and interleukin-1β (IL-1β: IL-1*2 at +3953) were stimulated in vitro with bacterial lipopolysaccharide (LPS) and compared with tissues carrying the common alleles (TNF*1 and IL-1*1). Fetal membranes carrying the TNF*1 allele displayed an identical dose-response pattern to tissues carrying a TNF*2 allele, except at the highest dose of LPS tested (50 ng/ml) there was a significantly greater production of TNF-α in the presence of a TNF*2 allele. Membranes carrying the IL-1*2 polymorphism secreted IL-1β in a dose-response curve that was different from IL-1* tissues when challenged with 5, 10 and 50 ng/ml LPS. These observations support the hypothesis that reproductive tissues carrying hyper-responsive proinflammatory cytokine genes may over-respond to intrauterine infection secreting higher amounts of cytokines, which in turn, may lead to adverse pregnancy outcomes.
AB - The identification of polymorphisms in genes encoding proinflammatory cytokines that affect transcription or the secretion rate has opened new ways to understand the variation in responses to infection during pregnancy. In this study, human amniochorion carrying hyper-responsive alleles of tumour necrosis factor-α (TNF-α: TNF*2 at -308) and interleukin-1β (IL-1β: IL-1*2 at +3953) were stimulated in vitro with bacterial lipopolysaccharide (LPS) and compared with tissues carrying the common alleles (TNF*1 and IL-1*1). Fetal membranes carrying the TNF*1 allele displayed an identical dose-response pattern to tissues carrying a TNF*2 allele, except at the highest dose of LPS tested (50 ng/ml) there was a significantly greater production of TNF-α in the presence of a TNF*2 allele. Membranes carrying the IL-1*2 polymorphism secreted IL-1β in a dose-response curve that was different from IL-1* tissues when challenged with 5, 10 and 50 ng/ml LPS. These observations support the hypothesis that reproductive tissues carrying hyper-responsive proinflammatory cytokine genes may over-respond to intrauterine infection secreting higher amounts of cytokines, which in turn, may lead to adverse pregnancy outcomes.
KW - Chorioamnion
KW - IL-1 gene polymorphism
KW - Infection during pregnancy
KW - Preterm labour
KW - TNF-α gene polymorphism
UR - http://www.scopus.com/inward/record.url?scp=0141728377&partnerID=8YFLogxK
U2 - 10.1093/molehr/gag076
DO - 10.1093/molehr/gag076
M3 - Artículo
SN - 1360-9947
VL - 9
SP - 625
EP - 629
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 10
ER -