TY - JOUR
T1 - Immunochemistry of type XIV pneumococcus capsular polysaccharide oxidized by d-galactose oxidase
AU - Estrada-Parra, Sergio
AU - Gómez, Irma
N1 - Funding Information:
*Carried out in part with the aid of a grant from Research Corporation. Presented in part before the X International Congress of Microbiology. tFellow of the C.O.F.A.A. of the I.P.N.
PY - 1972/11
Y1 - 1972/11
N2 - Type XIV pneumococcus specific capsular polysaccharide, SXIV, is made of a main chain of d-galactose and N-acetyl glucosamine and three types of side chain residues: one consists of d-glucose and the other two consist one of alpha d-galactose and the other of lactose, containing beta galactose in the terminal end. Under certain conditions, d-galactose oxidase can attack one or two of these terminal galactoses, oxidazing the hydroxy groups in position six to adelhydes. Further oxidation to carboxyl groups can be obtained by treatment with sodium chlorite in acidic conditions. By variations of these procedures three different derivatives of SXIV can be obtained which precipitate different amounts of antibody from an anti-SXIV horse serum: SXIV untreated, precipitates 650 μg of antibody N/ml; SXIV with one galactose oxidized to adelhyde precipitates 641 μg; SXIV with two galactoses oxidized to adelhydes precipitates 603 μg; SXIV with two galactoses converted to galacturonic acid precipitates 500 μg, and SXIV oxidized with periodate precipitates 274 μg. SXIV with two terminal galacturonic acid residues precipitates also in antipneumococcus Type I horse serum. The internal galactoses in the main chain are not attacked by the enzyme. The aldehyde groups can be reduced to alcohol again with NaBH4 without loss of immunological specificity with respect to untreated SXIV.
AB - Type XIV pneumococcus specific capsular polysaccharide, SXIV, is made of a main chain of d-galactose and N-acetyl glucosamine and three types of side chain residues: one consists of d-glucose and the other two consist one of alpha d-galactose and the other of lactose, containing beta galactose in the terminal end. Under certain conditions, d-galactose oxidase can attack one or two of these terminal galactoses, oxidazing the hydroxy groups in position six to adelhydes. Further oxidation to carboxyl groups can be obtained by treatment with sodium chlorite in acidic conditions. By variations of these procedures three different derivatives of SXIV can be obtained which precipitate different amounts of antibody from an anti-SXIV horse serum: SXIV untreated, precipitates 650 μg of antibody N/ml; SXIV with one galactose oxidized to adelhyde precipitates 641 μg; SXIV with two galactoses oxidized to adelhydes precipitates 603 μg; SXIV with two galactoses converted to galacturonic acid precipitates 500 μg, and SXIV oxidized with periodate precipitates 274 μg. SXIV with two terminal galacturonic acid residues precipitates also in antipneumococcus Type I horse serum. The internal galactoses in the main chain are not attacked by the enzyme. The aldehyde groups can be reduced to alcohol again with NaBH4 without loss of immunological specificity with respect to untreated SXIV.
UR - http://www.scopus.com/inward/record.url?scp=0015434602&partnerID=8YFLogxK
U2 - 10.1016/0019-2791(72)90079-1
DO - 10.1016/0019-2791(72)90079-1
M3 - Artículo
SN - 0019-2791
VL - 9
SP - 1095-1098,IN9,1099-1101
JO - Immunochemistry
JF - Immunochemistry
IS - 11
ER -