TY - JOUR
T1 - Identification of phosphatidate nonlamellar phases on liposomes by flow cytometry.
AU - Baeza, I.
AU - Aguilar, L.
AU - Alvarado-Alemán, F.
AU - Soto, C.
AU - Escobar-Gutiérrez, A.
AU - Mondragón, R.
AU - González, S.
AU - Ibáñez, M.
AU - Wong, C.
PY - 1995
Y1 - 1995
N2 - This study is the first report that demonstrates nonlamellar arrangements, or lipidic particles, of phosphatidate inserted in the lipid bilayer of liposomes using polyclonal antibodies from mice and flow cytometry. Sera immunoreactivity was analyzed using liposomes that displayed smooth bilayers of phosphatidate particles, as shown by electron microscopy. This cytofluorimetric analysis showed that immune mice sera have a specific immunoreactivity with the phosphatide particles formed by Mn2+, which also cross-reacted with those formed by Ca2+ and with cardiolipin particles formed by Mn2+. In addition, these immune sera hardly reacted with smooth bilayered liposomes, independently of the lipid composition studied. Thus, this new methodology can be applied to demonstrate nonlamellar molecular arrangements in biological membranes.
AB - This study is the first report that demonstrates nonlamellar arrangements, or lipidic particles, of phosphatidate inserted in the lipid bilayer of liposomes using polyclonal antibodies from mice and flow cytometry. Sera immunoreactivity was analyzed using liposomes that displayed smooth bilayers of phosphatidate particles, as shown by electron microscopy. This cytofluorimetric analysis showed that immune mice sera have a specific immunoreactivity with the phosphatide particles formed by Mn2+, which also cross-reacted with those formed by Ca2+ and with cardiolipin particles formed by Mn2+. In addition, these immune sera hardly reacted with smooth bilayered liposomes, independently of the lipid composition studied. Thus, this new methodology can be applied to demonstrate nonlamellar molecular arrangements in biological membranes.
UR - http://www.scopus.com/inward/record.url?scp=0029295645&partnerID=8YFLogxK
U2 - 10.1139/o95-036
DO - 10.1139/o95-036
M3 - Artículo
SN - 0829-8211
VL - 73
SP - 289
EP - 297
JO - Biochemistry and Cell Biology
JF - Biochemistry and Cell Biology
IS - 5-6
ER -