TY - JOUR
T1 - Identification of candidate genes for reproductive traits in cattle using a functional interaction network approach
AU - Paredes-Sánchez, Francisco Alejandro
AU - Trejo-Martínez, Daniel
AU - Herrera-Mayorga, Elsa Verónica
AU - Arellano-Vera, Williams
AU - Almeida, Felipe Rodríguez
AU - Sifuentes-Rincón, Ana María
N1 - Publisher Copyright:
© 2020 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Reproduction is a key element in cattle production systems. Systems biology approaches, including those involving gene networks, have been applied to genetic dissection complex phenotypes in cattle. A set of 385 genes associated with reproductive traits in cattle were included in a protein-protein network analysis to identify and prioritize candidate genes related to phenotypic differences in cattle reproduction. Genes belonging to the ubiquitin family - Ubiquitin C (Ubc, Gene ID: 444874) and Ubiquitin B (Ubb, Gene ID: 281370) -had the highest probability of being associated with these traits in cattle. Both proteins were identified as important hubs in a protein-protein interaction network, each having 3,775 interactions of 3,856 possible. Resequencing of the Ubb gene coding region to evaluate the presence of SNPs in a discovery population identified the G/T (rs110366695) transversion. This causes emergence of a stop codon and a protein truncated by 287 aa. The allelic frequency distributions found in two beef cattle breeds highlight the promise of further research into the effects of protein truncation and the potential of these proteins as molecular markers for semen quality.
AB - Reproduction is a key element in cattle production systems. Systems biology approaches, including those involving gene networks, have been applied to genetic dissection complex phenotypes in cattle. A set of 385 genes associated with reproductive traits in cattle were included in a protein-protein network analysis to identify and prioritize candidate genes related to phenotypic differences in cattle reproduction. Genes belonging to the ubiquitin family - Ubiquitin C (Ubc, Gene ID: 444874) and Ubiquitin B (Ubb, Gene ID: 281370) -had the highest probability of being associated with these traits in cattle. Both proteins were identified as important hubs in a protein-protein interaction network, each having 3,775 interactions of 3,856 possible. Resequencing of the Ubb gene coding region to evaluate the presence of SNPs in a discovery population identified the G/T (rs110366695) transversion. This causes emergence of a stop codon and a protein truncated by 287 aa. The allelic frequency distributions found in two beef cattle breeds highlight the promise of further research into the effects of protein truncation and the potential of these proteins as molecular markers for semen quality.
KW - Bovine
KW - Molecular markers
KW - Semen quality
KW - Ubiquitylation
UR - http://www.scopus.com/inward/record.url?scp=85092747515&partnerID=8YFLogxK
U2 - 10.22319/RMCP.V11I3.5279
DO - 10.22319/RMCP.V11I3.5279
M3 - Artículo
AN - SCOPUS:85092747515
SN - 2007-1124
VL - 11
SP - 894
EP - 904
JO - Revista Mexicana De Ciencias Pecuarias
JF - Revista Mexicana De Ciencias Pecuarias
IS - 3
ER -