Identificación Morfológica y Molecular de Barrenadores del Tallo de la Caña de Azúcar¹ Distribuidos en Sinaloa

Three species of sugarcane borers were identified based on study of the male genitalia; these were distributed at 28 sampling sites of northern and central sugarcane areas in Sinaloa, Mexico. The molecular identification of the three species was done by sequencing the enzyme gene of the subunit I of cytochrome c oxidase (COI) of the DNA mitochondrial. Diatraea considerata Heinrich, D. grandiosella Dyar, and Eoreuma loftini (Dyar) were identified with their distribution in north zone (Los Mochis) and central zone (El Dorado) of sugarcane area. The nucleotide sequences of these species were compared in GenBank, had a similarity to D. considerata 99% (673 bp) (acc. No. KP259614), D. grandiosella 97% (647 bp) (acc. no. KP259615), and E. loftini 99% (645 bp) (acc. no. KP259616). An Analysis of Maximum Likelihood (ML) was done to determine the proximity between these species, founding that the sequences correspond to these same species reported in GenBank, with a confidence of 96% to D. considerata, 88% to E. loftini, and 100% to D. grandiosella, giving certainty to the identification of these species distributed in this area. This information is basic for the implementation of future strategies for biological control of these pests in Sinaloa.