Identificación Molecular y Actividad Patogénica de Aislamientos de Beauveria bassiana contra Spodoptera frugiperda (Lepidoptera: Noctuidae)

Cipriano García Gutiérrez, Ninfa María Rosas García, Cosme Bojórquez Ramos, Jorge Soto Alcalá

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

Twenty two monosporic Beauveria bassiana isolates obtained from maize soil were identified by molecular techniques. Genomic DNA was amplified by PCR using the primers pair ITS1 and ITS4. These primers amplified for 550 bp fragment that includes part of the 18 S subunit, ITS1, 5.8 S subunit, ITS2, and 28 S subunit of the ribosomal DNA. The DNA was cloned with E coli JM 109, and then a new PCR was carried out using the M13F and M13R primers. After that, the DNA product was sequenced it was assessed by the NCBI BLAST program, resulting a homology of 99% with other species of B. bassiana in the GeneBank. Ten monosporic B. bassiana isolates were evaluated at 1.2 × 106 conidia/ml against neonate larvae of fall armyworm S. frugiperda. The better isolate of B. bassiana code CIDDB02 with assessed number KP860298 had LC50 of 2.3 × 107 conidia/ml, with 100% of insect mortality 72 h after conidia inoculation. This study allowed us to identify fast and reliably one B. bassiana isolated with highest pathogenic activity against the fall armyworm.

Título traducido de la contribuciónMolecular identification and pathogenic activity of Beauveria bassiana isolates against Spodoptera frugiperda (Lepidoptera: Noctuidae): Noctuidae)
Idioma originalEspañol
Páginas (desde-hasta)539-544
Número de páginas6
PublicaciónSouthwestern Entomologist
Volumen40
N.º3
DOI
EstadoPublicada - 1 sep. 2015

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