TY - JOUR
T1 - Identificación del genotipo Mycobacterium tuberculosis RDRio en el norte de México y su asociación con la resistencia a isoniazida
AU - Bocanegra-García, Virgilio
AU - Cortez-de-la-Fuente, Luis Jesús
AU - Nakamura-López, Yuko
AU - González, Gloria María
AU - Rivera, Gildardo
AU - Palma-Nicolás, José Prisco
N1 - Funding Information:
This work was partially supported by the Fondo Mixto (FOMIX) CONACYT-Gobierno del Estado de Oaxaca, Grant Number: 193298 to YNL, and the PRODEP Grant Number: DSA/103.5/14/10812 to JPPN. We also thanks to the National Council on Science and Technology (CONACyT) by the approval of the Biosafety Grant to Gloria M. Gonz?lez (Grant number: 264118 ?Fortalecimiento de la bioseguridad para el proceso de muestras biol?gicas con pat?genos del grupo de riesgo 3, en apoyo a actividades de investigaci?n, formaci?n de recursos humanos y servicio del Centro Regional de Control de Enfermedades Infecciosas, UANL?).
Funding Information:
This work was partially supported by the Fondo Mixto (FOMIX) CONACYT-Gobierno del Estado de Oaxaca , Grant Number: 193298 to YNL, and the PRODEP Grant Number: DSA/103.5/14/10812 to JPPN. We also thanks to the National Council on Science and Technology (CONACyT) by the approval of the Biosafety Grant to Gloria M. González (Grant number: 264118 “Fortalecimiento de la bioseguridad para el proceso de muestras biológicas con patógenos del grupo de riesgo 3, en apoyo a actividades de investigación, formación de recursos humanos y servicio del Centro Regional de Control de Enfermedades Infecciosas, UANL”).
Publisher Copyright:
© 2020 Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
PY - 2020
Y1 - 2020
N2 - Background: It has been established that the genomic background of Mycobacterium tuberculosis may influence disease progression, in particular for the Beijing family and the Latin American and Mediterranean (LAM)/RDRio strains. The purpose of this study was to evaluate the prevalence of the LAM/RDRio genotype in cases of tuberculosis from Mexico and their drug susceptibility profile. Methods: Two hundred eighteen M. tuberculosis isolates were screened by 43-spacer spoligotyping. The LAM/RDRio genotype was confirmed by multiplex PCR, and the drug susceptibility testing was carried out in solid Löwenstein-Jensen media. Results: Among the LAM strains identified, 24 (63.1%) were confirmed as M. tuberculosis RDRio. All RDRio strains shared the RD174 deletion, that was associated with isoniazid resistance (p = 0.0264). Conclusions: We documented for the first time the isolation of the LAM/RDRio genotype in pulmonary cases of tuberculosis in Mexico, and we found resistance to the first-line anti-tuberculosis drug isoniazid in these strains.
AB - Background: It has been established that the genomic background of Mycobacterium tuberculosis may influence disease progression, in particular for the Beijing family and the Latin American and Mediterranean (LAM)/RDRio strains. The purpose of this study was to evaluate the prevalence of the LAM/RDRio genotype in cases of tuberculosis from Mexico and their drug susceptibility profile. Methods: Two hundred eighteen M. tuberculosis isolates were screened by 43-spacer spoligotyping. The LAM/RDRio genotype was confirmed by multiplex PCR, and the drug susceptibility testing was carried out in solid Löwenstein-Jensen media. Results: Among the LAM strains identified, 24 (63.1%) were confirmed as M. tuberculosis RDRio. All RDRio strains shared the RD174 deletion, that was associated with isoniazid resistance (p = 0.0264). Conclusions: We documented for the first time the isolation of the LAM/RDRio genotype in pulmonary cases of tuberculosis in Mexico, and we found resistance to the first-line anti-tuberculosis drug isoniazid in these strains.
KW - Beijing
KW - Isonizid
KW - RD
KW - SIT163
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85089824371&partnerID=8YFLogxK
U2 - 10.1016/j.eimc.2020.07.003
DO - 10.1016/j.eimc.2020.07.003
M3 - Artículo
C2 - 32854960
AN - SCOPUS:85089824371
SN - 0213-005X
VL - 39
SP - 399
EP - 402
JO - Enfermedades Infecciosas y Microbiologia Clinica
JF - Enfermedades Infecciosas y Microbiologia Clinica
IS - 8
ER -
