TY - JOUR
T1 - Hypomethylation of ahrr (Cg05575921) is related to smoking status in the mexican mestizo population
AU - Bravo‐gutiérrez, Omar Andrés
AU - Falfán‐valencia, Ramcés
AU - Ramírez‐venegas, Alejandra
AU - Sansores, Raúl H.
AU - Hernández‐zenteno, Rafael de Jesús
AU - Hernández‐pérez, Andrea
AU - García‐gómez, Leonor
AU - Osio‐echánove, Jennifer
AU - Abarca‐rojano, Edgar
AU - Pérez‐rubio, Gloria
N1 - Publisher Copyright:
© 2021 by the author. Licensee MDPI, Basel, Switzerland.
PY - 2021/8
Y1 - 2021/8
N2 - Tobacco smoking results in a multifactorial disease involving environmental and genetic factors; epigenome‐wide association studies (EWAS) show changes in DNA methylation levels due to cigarette consumption, partially reversible upon tobacco smoking cessation. Therefore, methylation levels could predict smoking status. This study aimed to evaluate the DNA methylation level of cg05575921 (AHRR) and cg23771366 (PRSS23) and their correlation with lung function variables, cigarette consumption, and nicotine addiction in the Mexican smoking population. We included 114 non‐smokers (NS) and 102 current tobacco smokers (TS); we then further subclassified them as heavy smokers (HS) (n = 53) and light smokers (LS) (n = 49). We used restriction enzymes (MspI/HpaII) and qPCR to determine the DNA methylation level. We observed significant hypo-methylation of cg05575921 in smokers compared to NS (p = 0.003); further analysis found a difference between HS and NS (p = 0.02). We did not observe differences between other groups or a positive correlation between methylation levels and age, BMI, cigarette consumption, nicotine addiction, or lung function. In conclusion, the cg05575921 site of AHRR is significantly hypomethylated in Mexican smokers, especially in HS (≥20 cigarettes per day).
AB - Tobacco smoking results in a multifactorial disease involving environmental and genetic factors; epigenome‐wide association studies (EWAS) show changes in DNA methylation levels due to cigarette consumption, partially reversible upon tobacco smoking cessation. Therefore, methylation levels could predict smoking status. This study aimed to evaluate the DNA methylation level of cg05575921 (AHRR) and cg23771366 (PRSS23) and their correlation with lung function variables, cigarette consumption, and nicotine addiction in the Mexican smoking population. We included 114 non‐smokers (NS) and 102 current tobacco smokers (TS); we then further subclassified them as heavy smokers (HS) (n = 53) and light smokers (LS) (n = 49). We used restriction enzymes (MspI/HpaII) and qPCR to determine the DNA methylation level. We observed significant hypo-methylation of cg05575921 in smokers compared to NS (p = 0.003); further analysis found a difference between HS and NS (p = 0.02). We did not observe differences between other groups or a positive correlation between methylation levels and age, BMI, cigarette consumption, nicotine addiction, or lung function. In conclusion, the cg05575921 site of AHRR is significantly hypomethylated in Mexican smokers, especially in HS (≥20 cigarettes per day).
KW - AHRR
KW - Cg05575921
KW - Cg23771366
KW - DNA methylation
KW - PRSS23
KW - Tobacco smoking
UR - http://www.scopus.com/inward/record.url?scp=85113560818&partnerID=8YFLogxK
U2 - 10.3390/genes12081276
DO - 10.3390/genes12081276
M3 - Artículo
C2 - 34440450
AN - SCOPUS:85113560818
SN - 2073-4425
VL - 12
JO - Genes
JF - Genes
IS - 8
M1 - 1276
ER -