TY - JOUR
T1 - Hydrothermal kinetic modeling for microalgae biomass under subcritical condition cultivated in a close bubble tubular photobioreactor
AU - Rosero-Chasoy, Gilver
AU - Rodríguez-Jasso, Rosa M.
AU - Aguilar, Cristóbal N.
AU - Buitrón, Germán
AU - Chairez, Isaac
AU - Ruiz, Héctor A.
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/2/15
Y1 - 2023/2/15
N2 - In this study, the Spirulina platenesis growth and carbohydrates, proteins, lipids, and chlorophyll production were investigated by using synthetic wastewater via a mixture experimental design using carbon–nitrogen–phosphorus (C:N:P) relation according to the mixing obtained in the experimental space. In addition, a scale-up in the growth of microalgal biomass in a tubular column photobioreactor was carried out. The activation energy was determined by thermogravimetric analysis from the Spirulina platenesis biomass obtained from the experimental design. Also, kinetic growth analysis and thermal hydrolysis of Spirulina platensis biomass were performed for the experimental case where the carbohydrate concentrations were the highest. The stress induced by phosphorus limitation in the synthetic wastewater enhanced intracellular carbohydrates production. It achieves a maximum concentration of 859.59 ± 80.87 mg/L (60.11% w/w) on run 8. The results showed a relation between the activation energy and carbohydrates concentration in biomass. This indicates that the energy required to start the thermal degradation reaction increases as carbohydrate concentration increases. In terms of hydrothermal pretreatment, the temperature increment in the reactor raises the condition to extract carbohydrates from microalgae biomass. In contrast, the temperature increment decreases the reaction rate on the protein extraction. During the hydrothermal pretreatment, the best condition for the carbohydrates extraction in the Spirulina platensis biomass was 140oC for 45 min. At this condition, cell wall hydrolysis needs an energy supply of 18.65 kJ/mol. The development of this process will allow the fractionation of microalgal biomass and extraction of carbohydrates in terms of a biorefinery.
AB - In this study, the Spirulina platenesis growth and carbohydrates, proteins, lipids, and chlorophyll production were investigated by using synthetic wastewater via a mixture experimental design using carbon–nitrogen–phosphorus (C:N:P) relation according to the mixing obtained in the experimental space. In addition, a scale-up in the growth of microalgal biomass in a tubular column photobioreactor was carried out. The activation energy was determined by thermogravimetric analysis from the Spirulina platenesis biomass obtained from the experimental design. Also, kinetic growth analysis and thermal hydrolysis of Spirulina platensis biomass were performed for the experimental case where the carbohydrate concentrations were the highest. The stress induced by phosphorus limitation in the synthetic wastewater enhanced intracellular carbohydrates production. It achieves a maximum concentration of 859.59 ± 80.87 mg/L (60.11% w/w) on run 8. The results showed a relation between the activation energy and carbohydrates concentration in biomass. This indicates that the energy required to start the thermal degradation reaction increases as carbohydrate concentration increases. In terms of hydrothermal pretreatment, the temperature increment in the reactor raises the condition to extract carbohydrates from microalgae biomass. In contrast, the temperature increment decreases the reaction rate on the protein extraction. During the hydrothermal pretreatment, the best condition for the carbohydrates extraction in the Spirulina platensis biomass was 140oC for 45 min. At this condition, cell wall hydrolysis needs an energy supply of 18.65 kJ/mol. The development of this process will allow the fractionation of microalgal biomass and extraction of carbohydrates in terms of a biorefinery.
KW - Biomass fractionation
KW - Biorefinery
KW - Circular bioeconomy
KW - Kinetic modeling
KW - Spirulina platenesis
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=85142122898&partnerID=8YFLogxK
U2 - 10.1016/j.fuel.2022.126585
DO - 10.1016/j.fuel.2022.126585
M3 - Artículo
AN - SCOPUS:85142122898
SN - 0016-2361
VL - 334
JO - Fuel
JF - Fuel
M1 - 126585
ER -