TY - JOUR
T1 - HPLC method for quantification of oxidative stress by salicilate hydroxylation in human plasma
AU - Lazalde-Ramos, Blanca P.
AU - Lares-Asseff, Ismael
AU - Villanueva-Fierro, Ignacio
AU - Sosa-Macías, Martha
AU - Yahuaca-Mendoza, Patricia
AU - Zamora-Perez, Ana
N1 - Funding Information:
This work was financially supported by CONACyT (grant number: G34049-M). Blanca P. Lazalde Ramos is a fellow from CONACyT México (number 179418) during Ph.D. studies.
PY - 2010/9
Y1 - 2010/9
N2 - The aim of the present study was to modify and validate a highperformance liquid chromatographic (HPLC) method for determining 2,3 and 2,5 di hydroxy benzoic acid (2,3-DHBA and 2,5-DHBA) from salicylic acid in human plasma. The mobile phase was a mixture of sodium acetate/citrate (pH 2.5) 30 mM-methanol (93:7, v/v). The injection volume was 10 μL. Retention time for 2,5-DHBA, and 2,3-DHBA was 4.5 ± 0.10 and 5.8 ± 0.15 min, respectively. The detection and quantification limits were 10 and 40 nM for 2,3-DHBA and 8 and 20 nM for 2,5-DHBA. Linearity was evaluated in the range of 40-1600 nM for both metabolites. Interand intra-analysis variation coefficient was below 10%. Good recoveries of more than 99% were obtained for both metabolites using this method.
AB - The aim of the present study was to modify and validate a highperformance liquid chromatographic (HPLC) method for determining 2,3 and 2,5 di hydroxy benzoic acid (2,3-DHBA and 2,5-DHBA) from salicylic acid in human plasma. The mobile phase was a mixture of sodium acetate/citrate (pH 2.5) 30 mM-methanol (93:7, v/v). The injection volume was 10 μL. Retention time for 2,5-DHBA, and 2,3-DHBA was 4.5 ± 0.10 and 5.8 ± 0.15 min, respectively. The detection and quantification limits were 10 and 40 nM for 2,3-DHBA and 8 and 20 nM for 2,5-DHBA. Linearity was evaluated in the range of 40-1600 nM for both metabolites. Interand intra-analysis variation coefficient was below 10%. Good recoveries of more than 99% were obtained for both metabolites using this method.
UR - http://www.scopus.com/inward/record.url?scp=77958009444&partnerID=8YFLogxK
U2 - 10.1093/chromsci/48.8.675
DO - 10.1093/chromsci/48.8.675
M3 - Artículo
SN - 0021-9665
VL - 48
SP - 675
EP - 679
JO - Journal of chromatographic science
JF - Journal of chromatographic science
IS - 8
ER -