TY - JOUR
T1 - Honey characterization and identification of fructophilic lactic acid bacteria of fresh samples from Melipona beecheii, Scaptotrigona pectoralis, Plebeia llorentei, and Plebeia jatiformis hives
AU - Andrade-Velásquez, Amaury
AU - Hernández Sánchez, Humberto
AU - Dorantes-Álvarez, Lidia
AU - Palmeros-Sánchez, Beatriz
AU - Torres-Moreno, Raymundo
AU - Hernández-Rodríguez, Dolores
AU - Melgar-Lalanne, Guiomar
N1 - Publisher Copyright:
Copyright © 2023 Andrade-Velásquez, Hernández Sánchez, Dorantes-Álvarez, Palmeros-Sánchez, Torres-Moreno, Hernández-Rodríguez and Melgar-Lalanne.
PY - 2023
Y1 - 2023
N2 - Stingless bees are essential to preser tropical ecosystems. They pollinate native flora, producing honey with properties for traditional health uses. Lactic acid bacteria spontaneously ferment honey in stingless bee honey (SBH). This study aims to determine the main physicochemical characteristics of Melipona beecheii, Scraptotrigona pectoralis, Plebeia jatiformis and Plebeia llorentei honey and to isolate and identify FLAB present in SBH samples. The physicochemical properties of SBH, such as color, pH, acidity, sugars, protein, total soluble solids, water activity, total polyphenols, and antioxidant activity, were determined since these parameters can be related to the presence of some bacteria groups, and with health benefits for humans and the hive ecosystems. FLAB harvested from honey, taken directly from storing pots of the hives, were identified by 16S ribosomal RNA sequencing and preserved for future biotechnological use due to their resistance to non-ionic osmotic stress. The results showed significant differences in the physicochemical characteristics of SBH samples. Seven FLAB from four stingless bee species were identified as Fructobacillus pseudoficulneus and F. tropaeoli. In addition, three other strains of Fructilactobacillus spp. were identified only at the genus level. All species showed the ability to grow under different carbon sources, resulting in negative hemolysis and sensitivity to cefuroxime, erythromycin, and chloramphenicol. To the best of our knowledge, this is the first time that the physicochemical and FLAB characterization of SBH from P. jatiformis and P. llorentei has been reported. Therefore, the future following research should be focused on the environmental, health and food biotechnological applications implications of FLAB from SBH.
AB - Stingless bees are essential to preser tropical ecosystems. They pollinate native flora, producing honey with properties for traditional health uses. Lactic acid bacteria spontaneously ferment honey in stingless bee honey (SBH). This study aims to determine the main physicochemical characteristics of Melipona beecheii, Scraptotrigona pectoralis, Plebeia jatiformis and Plebeia llorentei honey and to isolate and identify FLAB present in SBH samples. The physicochemical properties of SBH, such as color, pH, acidity, sugars, protein, total soluble solids, water activity, total polyphenols, and antioxidant activity, were determined since these parameters can be related to the presence of some bacteria groups, and with health benefits for humans and the hive ecosystems. FLAB harvested from honey, taken directly from storing pots of the hives, were identified by 16S ribosomal RNA sequencing and preserved for future biotechnological use due to their resistance to non-ionic osmotic stress. The results showed significant differences in the physicochemical characteristics of SBH samples. Seven FLAB from four stingless bee species were identified as Fructobacillus pseudoficulneus and F. tropaeoli. In addition, three other strains of Fructilactobacillus spp. were identified only at the genus level. All species showed the ability to grow under different carbon sources, resulting in negative hemolysis and sensitivity to cefuroxime, erythromycin, and chloramphenicol. To the best of our knowledge, this is the first time that the physicochemical and FLAB characterization of SBH from P. jatiformis and P. llorentei has been reported. Therefore, the future following research should be focused on the environmental, health and food biotechnological applications implications of FLAB from SBH.
KW - 16S ribosomal RNA
KW - antioxidant properties
KW - fructophilic lactic acid bacteria
KW - honey characterization
KW - stingless bees
KW - stingless honey
UR - http://www.scopus.com/inward/record.url?scp=85150747215&partnerID=8YFLogxK
U2 - 10.3389/fsufs.2023.1113920
DO - 10.3389/fsufs.2023.1113920
M3 - Artículo
AN - SCOPUS:85150747215
SN - 2571-581X
VL - 7
JO - Frontiers in Sustainable Food Systems
JF - Frontiers in Sustainable Food Systems
M1 - 1113920
ER -