TY - JOUR
T1 - High Serum Levels of High-Mobility Group Box 1 (HMGB1) and Low Levels of Heat Shock Protein 70 (Hsp70) are Associated with Poor Prognosis in Patients with Acute Pancreatitis
AU - Arriaga-Pizano, Lourdes
AU - Boscó-Gárate, Ilka
AU - Martínez-Ordaz, José Luis
AU - Wong-Baeza, Isabel
AU - Gutiérrez-Mendoza, Mireille
AU - Sánchez-Fernandez, Patricio
AU - López-Macías, Constantino
AU - Isibasi, Armando
AU - Pelaez-Luna, Mario
AU - Cérbulo-Vázquez, Arturo
AU - Torres-González, Rubén
AU - Ferat-Osorio, Eduardo
N1 - Publisher Copyright:
© 2019
PY - 2018/10
Y1 - 2018/10
N2 - Introduction: Cell damage in Acute Pancreatitis (AP) lead to release of cytokines and HMGB1 and Hsp70. While Hsp70 plays a role in cytoprotection, when released to extracellular milieu constitutes, as HMGB1, a danger signal and trigger pro-inflammatory responses. These molecules seem to be related to the clinical progression; but because no evidence exists about them as molecular network in AP development, we quantify HSP70, HMGB1, and cytokines in patients with AP and search for correlations with severity and prognosis. Methods: Fifteen patients with AP were included. The average age was 52 years. Six patients had mild pancreatitis, 4 were moderately severe and 5 with a severe form. Blood samples were taken within the first 24 h, at 3d and 7d from the start. Serum HMGB1 and Hsp70 were determined using ELISA; TNF-α, IL-1β, IL-6, IL-8, IL-10 and IL-12p70 were determined by bead based immuassay. Results: Of all 15 patients recruited, 4 were women. Eight patients had APACHEII score higher than 8. Two patients died from AP related complications. Increase in serum HMGB1 and decrease of Hsp70 were associated with the severity and mortality. TNF-α, IL-6 and IL-8 were higher in patients that did not survive, in those with an APACHE II >8, and in those with severe AP. Conclusions: High HMGB1 and low Hsp70 were associated with poor prognosis. Hsp70 might play a protective role in AP. TNF-α, IL-6, IL-8, HMGB1 and Hsp70 during hospital admissions might serve to evaluate risk of death due to AP.
AB - Introduction: Cell damage in Acute Pancreatitis (AP) lead to release of cytokines and HMGB1 and Hsp70. While Hsp70 plays a role in cytoprotection, when released to extracellular milieu constitutes, as HMGB1, a danger signal and trigger pro-inflammatory responses. These molecules seem to be related to the clinical progression; but because no evidence exists about them as molecular network in AP development, we quantify HSP70, HMGB1, and cytokines in patients with AP and search for correlations with severity and prognosis. Methods: Fifteen patients with AP were included. The average age was 52 years. Six patients had mild pancreatitis, 4 were moderately severe and 5 with a severe form. Blood samples were taken within the first 24 h, at 3d and 7d from the start. Serum HMGB1 and Hsp70 were determined using ELISA; TNF-α, IL-1β, IL-6, IL-8, IL-10 and IL-12p70 were determined by bead based immuassay. Results: Of all 15 patients recruited, 4 were women. Eight patients had APACHEII score higher than 8. Two patients died from AP related complications. Increase in serum HMGB1 and decrease of Hsp70 were associated with the severity and mortality. TNF-α, IL-6 and IL-8 were higher in patients that did not survive, in those with an APACHE II >8, and in those with severe AP. Conclusions: High HMGB1 and low Hsp70 were associated with poor prognosis. Hsp70 might play a protective role in AP. TNF-α, IL-6, IL-8, HMGB1 and Hsp70 during hospital admissions might serve to evaluate risk of death due to AP.
KW - Acude pancreatitis
KW - Cytokines
KW - HMGB1
KW - Hsp70
UR - http://www.scopus.com/inward/record.url?scp=85063566419&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2019.02.003
DO - 10.1016/j.arcmed.2019.02.003
M3 - Artículo
C2 - 30947809
AN - SCOPUS:85063566419
SN - 0188-4409
VL - 49
SP - 504
EP - 511
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 7
ER -