TY - JOUR
T1 - Heat shock protein 60 from Klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone
AU - Ortega-Ortega, Yolanda
AU - García-Latorre, Ethel Awilda
AU - Lezama, Ruth Angélica
AU - Luna-Herrera, Julieta
AU - Vega-López, Armando
AU - Domínguez-López, María Lilia
N1 - Funding Information:
This work was supported in part by grants from SIP-IPN 20091228 and 20101054 . Y. Ortega-Ortega. DSc student (CQB), ENCB, IPN and are fellow of CONACyT and PIFI-IPN, ∗EA. García-Latorre PhD. Professor of Immunology, ENCB, IPN, ∗RA Lezama. DSc. Professor of Cellular Biology, ENCB, IPN, ∗J Luna-Herrera. DSc. Professor of Immunology, ENCB, IPN, ∗A Vega-López. DSc. Professor of Immunotoxicology, ENCB, IPN, ∗ML. Domínguez-López. DSc. Professor of Immunology, ENCB, IPN, ∗Authors are fellows of COFAA-IPN, EDI and SNI.
PY - 2011/11
Y1 - 2011/11
N2 - Aims: Bacterial heat shock proteins can have anti-apoptotic effects on human cells. We investigated whether enterobacterial HSP60 can protect peripheral blood mononuclear cells (PBMC) from DXM-induced apoptosis and if this effect requires cytoskeleton participation. Main methods: Anti-apoptotic effect from enterobacterial HSP60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before DXM induction. Percentage of apoptotic cells was determined by SubG0 peak and TUNEL techniques in a flow cytometer. Key findings: Our results showed significant protective effect of HSP60 Klebsiella pneumoniae and E. coli, in the DXM-induced apoptosis in PBMC. Similar results were obtained with recombinant human HSP60. The same protective effect of proteins was observed in CD4+ and CD8 + T cell subpopulations. To analyze if enterobacterial HSP60 need internalization to have the anti-apoptotic effect, we used cytoskeleton inhibitors such as: nocodazole, cytochalasin D and amiloride, the three inhibitors significantly affected the protective role of HSP60 in apoptosis induced with DXM. Results suggest that the protective effect of HSP60 K. pneumoniae and E. coli requires the participation of contractile structures for the internalization of this protein by the cells, we suggest that the internalization of enterobacterial HSP60 could be carry out by macropinocytosis. Significance: We report for the first time that K. pneumoniae and E. coli HSP60 have protective effect in the apoptosis induced with DXM in PBMC from healthy subjects and that this effect requires the internalization of the protein with active participation of the cytoskeleton.
AB - Aims: Bacterial heat shock proteins can have anti-apoptotic effects on human cells. We investigated whether enterobacterial HSP60 can protect peripheral blood mononuclear cells (PBMC) from DXM-induced apoptosis and if this effect requires cytoskeleton participation. Main methods: Anti-apoptotic effect from enterobacterial HSP60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before DXM induction. Percentage of apoptotic cells was determined by SubG0 peak and TUNEL techniques in a flow cytometer. Key findings: Our results showed significant protective effect of HSP60 Klebsiella pneumoniae and E. coli, in the DXM-induced apoptosis in PBMC. Similar results were obtained with recombinant human HSP60. The same protective effect of proteins was observed in CD4+ and CD8 + T cell subpopulations. To analyze if enterobacterial HSP60 need internalization to have the anti-apoptotic effect, we used cytoskeleton inhibitors such as: nocodazole, cytochalasin D and amiloride, the three inhibitors significantly affected the protective role of HSP60 in apoptosis induced with DXM. Results suggest that the protective effect of HSP60 K. pneumoniae and E. coli requires the participation of contractile structures for the internalization of this protein by the cells, we suggest that the internalization of enterobacterial HSP60 could be carry out by macropinocytosis. Significance: We report for the first time that K. pneumoniae and E. coli HSP60 have protective effect in the apoptosis induced with DXM in PBMC from healthy subjects and that this effect requires the internalization of the protein with active participation of the cytoskeleton.
KW - Apoptosis
KW - Cytoskeleton
KW - Dexamethasone
KW - Heath shock protein
KW - Mononuclear cells
UR - http://www.scopus.com/inward/record.url?scp=80052503388&partnerID=8YFLogxK
U2 - 10.1016/j.micpath.2011.07.001
DO - 10.1016/j.micpath.2011.07.001
M3 - Artículo
C2 - 21791241
SN - 0882-4010
VL - 51
SP - 352
EP - 359
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
IS - 5
ER -