TY - JOUR
T1 - Gold nanoparticles
T2 - uptake in human mast cells and effect on cell viability, inflammatory mediators, and proliferation
AU - Gutiérrez-Calleja, Ramón A.
AU - Rodríguez-Cortés, Octavio
AU - Flores-Mejía, Raúl
AU - Muñoz-Diosdado, Alejandro
N1 - Publisher Copyright:
© 2021, The Korean Society of Toxicogenomics and Toxicoproteomics.
PY - 2021/10
Y1 - 2021/10
N2 - Background: Gold nanoparticles (AuNPs) have potential for a wide range of applications as therapeutic and diagnostic agents. Since they have a high probability of interacting with human immune cells, cytotoxicity studies must be conducted. The investigation of AuNP/immune cell interaction has mainly focused on macrophages and dendritic cells, along with some other cell lineages. Scarce information is available regarding the effect of AuNPs on mast cells, which are abundant in the skin, mucosa, and perivascular space. Objective: To examine the uptake of AuNPs by HMC-1 human mast cells and the resulting effect on cell viability, pro-inflammatory mediators production, and proliferation. Results: With AuNPs treatment, the viability of HMC-1 cells decreased slightly (never less than 95%) during the first 4 h, but no changes were detected in the proliferation rate at any time. Increasing concentrations of AuNPs produced greater cell granularity (uptake). CLSM images exhibited AuNPs clusters in the cell cytoplasm. TNF-α and ROS production was not stimulated by AuNPs treatment at any concentration/time. Conclusion: Internalization of AuNPs into HMC-1 cells was demonstrated in an in vitro model, without showing cytotoxic effects or induction of pro-inflammatory mediators at any concentration tested.
AB - Background: Gold nanoparticles (AuNPs) have potential for a wide range of applications as therapeutic and diagnostic agents. Since they have a high probability of interacting with human immune cells, cytotoxicity studies must be conducted. The investigation of AuNP/immune cell interaction has mainly focused on macrophages and dendritic cells, along with some other cell lineages. Scarce information is available regarding the effect of AuNPs on mast cells, which are abundant in the skin, mucosa, and perivascular space. Objective: To examine the uptake of AuNPs by HMC-1 human mast cells and the resulting effect on cell viability, pro-inflammatory mediators production, and proliferation. Results: With AuNPs treatment, the viability of HMC-1 cells decreased slightly (never less than 95%) during the first 4 h, but no changes were detected in the proliferation rate at any time. Increasing concentrations of AuNPs produced greater cell granularity (uptake). CLSM images exhibited AuNPs clusters in the cell cytoplasm. TNF-α and ROS production was not stimulated by AuNPs treatment at any concentration/time. Conclusion: Internalization of AuNPs into HMC-1 cells was demonstrated in an in vitro model, without showing cytotoxic effects or induction of pro-inflammatory mediators at any concentration tested.
KW - Cytotoxicity
KW - Flow cytometry
KW - Gold nanoparticles
KW - Human mast cells
KW - Proliferation
KW - ROS
KW - TNF-α
KW - Uptake
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85115130972&partnerID=8YFLogxK
U2 - 10.1007/s13273-021-00152-7
DO - 10.1007/s13273-021-00152-7
M3 - Artículo
AN - SCOPUS:85115130972
SN - 1738-642X
VL - 17
SP - 439
EP - 452
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
IS - 4
ER -