TY - JOUR
T1 - Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes
AU - Romero-Nava, Rodrigo
AU - Alarcón-Aguilar, Francisco J.
AU - Giacoman-Martínez, Abraham
AU - Blancas-Flores, Gerardo
AU - Aguayo-Cerón, Karla A.
AU - Ballinas-Verdugo, Martha A.
AU - Sánchez-Muñoz, Fausto
AU - Huang, Fengyang
AU - Villafaña-Rauda, Santiago
AU - Almanza-Pérez, Julio C.
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2021/5
Y1 - 2021/5
N2 - Objective: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells. Methods: RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA. Results: Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor. Conclusion: These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.
AB - Objective: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells. Methods: RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA. Results: Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor. Conclusion: These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.
KW - Adipokines
KW - Glycine receptor
KW - Inflammation
KW - TNF-α receptor
KW - siRNA
UR - http://www.scopus.com/inward/record.url?scp=85105011628&partnerID=8YFLogxK
U2 - 10.1007/s00011-021-01462-1
DO - 10.1007/s00011-021-01462-1
M3 - Artículo
C2 - 33877377
AN - SCOPUS:85105011628
SN - 1023-3830
VL - 70
SP - 605
EP - 618
JO - Inflammation Research
JF - Inflammation Research
IS - 5
ER -