TY - JOUR
T1 - Genotoxic and cytotoxic effects produced by acetogenins obtained from Annona cherimolia Mill.
AU - García-Aguirre, Karol Karla
AU - Zepeda-Vallejo, Luis Gerardo
AU - Ramón-Gallegos, Eva
AU - Alvárez-González, Isela
AU - Madrigal-Bujaidar, Eduardo
PY - 2008
Y1 - 2008
N2 - In the present study we extracted three isomeric acetogenins (Ace) from the seeds of Annona cherimolia MILL. (Annonaceae) and determined their genotoxic and cytotoxic potential in mice. Our results showed a significant increase in the rate of micronucleated polychromatic erythrocytes induced by Ace with respect to the value for the control group; the effect was less pronounced than that observed with daunorubicin (Dau). To evaluate cytotoxicity, we determined the proportion of polychromatic erythrocytes with respect to the number of normochromatic erythrocytes, and we found an inhibitory effect induced by Ace that was quite similar to that observed with Dau. Besides, by means of the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test, we also determined the cytotoxicity of Ace in both a normal fibroblast mouse cell line and in a line derived from human colon cancer. In this assay, the strongest decrease in viability corresponded to the malignant cell line. Our results established for the first time the genotoxic capacity of these compounds in vivo, and confirmed their cytotoxic potential in cultured cells.
AB - In the present study we extracted three isomeric acetogenins (Ace) from the seeds of Annona cherimolia MILL. (Annonaceae) and determined their genotoxic and cytotoxic potential in mice. Our results showed a significant increase in the rate of micronucleated polychromatic erythrocytes induced by Ace with respect to the value for the control group; the effect was less pronounced than that observed with daunorubicin (Dau). To evaluate cytotoxicity, we determined the proportion of polychromatic erythrocytes with respect to the number of normochromatic erythrocytes, and we found an inhibitory effect induced by Ace that was quite similar to that observed with Dau. Besides, by means of the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test, we also determined the cytotoxicity of Ace in both a normal fibroblast mouse cell line and in a line derived from human colon cancer. In this assay, the strongest decrease in viability corresponded to the malignant cell line. Our results established for the first time the genotoxic capacity of these compounds in vivo, and confirmed their cytotoxic potential in cultured cells.
KW - Acetogenin
KW - Annona cherimolia
KW - Cytotoxicity
KW - Genotoxicity
KW - Micronuclei
UR - http://www.scopus.com/inward/record.url?scp=57349086296&partnerID=8YFLogxK
U2 - 10.1248/bpb.31.2346
DO - 10.1248/bpb.31.2346
M3 - Artículo
SN - 0918-6158
VL - 31
SP - 2346
EP - 2349
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 12
ER -