TY - JOUR
T1 - Genomic fingerprinting versus nuclear gene sequences
T2 - A comparative approach for studying the Lupinus montanus (Fabaceae) species complex
AU - Ferval, Maxime
AU - Legal, Luc
AU - Gers, Charles
AU - Winterton, Peter
AU - Bermúdez-Torres, Kalina
N1 - Funding Information:
The authors thank CONACYT ( 100808 ) and University Paul Sabatier for financial support, Céline Pélissier and Léa Bardon for technical advice.
PY - 2013/11
Y1 - 2013/11
N2 - The taxonomy and systematics of Mexican Lupinus are lacking in resolution, because the taxa are distinguished using a few minor and inconsistent morphological characters. The use of molecular markers can contribute to resolving such issues. In this study, we focused on two varieties of the Lupinus montanus complex (Fabaceae) in Mexico, L. montanus subsp. montanus var. montanus and L. montanus subsp. montanus var. nelsonii, and aimed to determine the most suitable genetic markers for clarifying taxon delimitation based on morphology. We compared hypervariable Inter Simple Sequence Repeats (ISSR) to gene sequences (External Transcribed Spacer (ETS) and Conserved Orthologous Sets (COS)).Distance analysis (ISSR) and maximum likelihood (ETS. +. COS) generated congruent results but with much more variability and resolution for ISSR. These data confirm the potential of ISSR for working at low taxonomic level and their reliability compared to that of gene sequences.
AB - The taxonomy and systematics of Mexican Lupinus are lacking in resolution, because the taxa are distinguished using a few minor and inconsistent morphological characters. The use of molecular markers can contribute to resolving such issues. In this study, we focused on two varieties of the Lupinus montanus complex (Fabaceae) in Mexico, L. montanus subsp. montanus var. montanus and L. montanus subsp. montanus var. nelsonii, and aimed to determine the most suitable genetic markers for clarifying taxon delimitation based on morphology. We compared hypervariable Inter Simple Sequence Repeats (ISSR) to gene sequences (External Transcribed Spacer (ETS) and Conserved Orthologous Sets (COS)).Distance analysis (ISSR) and maximum likelihood (ETS. +. COS) generated congruent results but with much more variability and resolution for ISSR. These data confirm the potential of ISSR for working at low taxonomic level and their reliability compared to that of gene sequences.
KW - COS
KW - ETS
KW - ISSR
KW - L. montanus
KW - Mexico
KW - Nuclear markers
KW - Trans-Mexican Volcanic Belt
UR - http://www.scopus.com/inward/record.url?scp=84887819295&partnerID=8YFLogxK
U2 - 10.1016/j.sajb.2013.06.021
DO - 10.1016/j.sajb.2013.06.021
M3 - Artículo
SN - 0254-6299
VL - 89
SP - 106
EP - 110
JO - South African Journal of Botany
JF - South African Journal of Botany
ER -