TY - JOUR
T1 - Expression of immune modulator cytokines in human fulminant amoebic colitis
AU - Sierra-Puente, R. E.
AU - Campos-RodrÍguez, R.
AU - Jarillo-Luna, R. A.
AU - MuÑoz-FernÁndez, L.
AU - RodrÍguez, M. G.
AU - MuÑoz-Ortega, M. H.
AU - Ventura-Juárez, J.
PY - 2009/7
Y1 - 2009/7
N2 - Summary Human fulminant amoebic colitis (FAC) is characterized by ulceration and inflammation of the colon. The specific mixture of pro-inflammatory and anti-inflammatory cytokines may participate in either the host defense or in the pathogenesis of amoebic colitis. Therefore, we studied the expression of IL-8, IL-10, IL-4, TGF-β and IFN-γ in human FAC patients and controls through immunohistochemistry analysis. The number of cells expressing IL-8, IL-4 and IL-10 was significantly enhanced in all FAC samples compared to the control samples. However, the expression of TGF- β in patients was low in the colonic mucosa and high in the lamina propria compared with the control. No expression of IFN-γ was found in the controls or FAC samples. The production of IL-8 by intestinal epithelial cells may play a role in the pathogenesis of amoebic infection, because this cytokine attracts neutrophils, which lead to an inflammatory reaction that results in tissue damage. The predominant expression of the macrophage down-regulating cytokines, IL-4, IL-10 and TGF-β, or the Th2-type immune response could inhibit a cell-mediated immune response, which in turn would facilitate parasite invasion in these tissues.
AB - Summary Human fulminant amoebic colitis (FAC) is characterized by ulceration and inflammation of the colon. The specific mixture of pro-inflammatory and anti-inflammatory cytokines may participate in either the host defense or in the pathogenesis of amoebic colitis. Therefore, we studied the expression of IL-8, IL-10, IL-4, TGF-β and IFN-γ in human FAC patients and controls through immunohistochemistry analysis. The number of cells expressing IL-8, IL-4 and IL-10 was significantly enhanced in all FAC samples compared to the control samples. However, the expression of TGF- β in patients was low in the colonic mucosa and high in the lamina propria compared with the control. No expression of IFN-γ was found in the controls or FAC samples. The production of IL-8 by intestinal epithelial cells may play a role in the pathogenesis of amoebic infection, because this cytokine attracts neutrophils, which lead to an inflammatory reaction that results in tissue damage. The predominant expression of the macrophage down-regulating cytokines, IL-4, IL-10 and TGF-β, or the Th2-type immune response could inhibit a cell-mediated immune response, which in turn would facilitate parasite invasion in these tissues.
KW - Cytokines
KW - Entamoeba histolytica
KW - Human fulminant amoebic colitis
UR - http://www.scopus.com/inward/record.url?scp=66949146894&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3024.2009.01118.x
DO - 10.1111/j.1365-3024.2009.01118.x
M3 - Artículo
C2 - 19527454
SN - 0141-9838
VL - 31
SP - 384
EP - 391
JO - Parasite Immunology
JF - Parasite Immunology
IS - 7
ER -