TY - JOUR
T1 - Expression and function of utrophin associated protein complex in stretched endothelial cells
T2 - Dissociation and activation of eNOS
AU - Ramirez-Sanchez, Israel
AU - Ceballos-Reyes, Guillermo
AU - Rosas-Vargas, Haydee
AU - Cerecedo-Mercado, Doris
AU - Zentella-Dehesa, Alejandro
AU - Salamanca, Fabio
AU - Coral-Vazquez, Ramon M.
PY - 2007
Y1 - 2007
N2 - Several studies have emphasized the relevance of dystrophin-associated protein complex (DAPC) to maintain the vascular function. Previously we postulated the presence of an utrophin associated protein complex (UAPC) in endothelium from umbilical cord vessels. In the present work, we demonstrate that utrophin (UTR) indeed forms a complex, with beta-dystroglycan (DG), epsilonsarcoglycan (SG), caveolin-1 (cav-1), and endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVEC) by co-immunoprecipitation analysis. Additionally, we observed an increment in the protein levels of epsilon-SG, beta-DC, UTR and cav-1 after mechanical stretching. Interestingly, this stimulus also induced eNOS up-regulation, activation and release from the UAPC, and led to a significant increase in nitric oxide (NO) production. Finally, we propose that UAPC in HUVECs may play an important role in the regulation of vascular tone.
AB - Several studies have emphasized the relevance of dystrophin-associated protein complex (DAPC) to maintain the vascular function. Previously we postulated the presence of an utrophin associated protein complex (UAPC) in endothelium from umbilical cord vessels. In the present work, we demonstrate that utrophin (UTR) indeed forms a complex, with beta-dystroglycan (DG), epsilonsarcoglycan (SG), caveolin-1 (cav-1), and endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVEC) by co-immunoprecipitation analysis. Additionally, we observed an increment in the protein levels of epsilon-SG, beta-DC, UTR and cav-1 after mechanical stretching. Interestingly, this stimulus also induced eNOS up-regulation, activation and release from the UAPC, and led to a significant increase in nitric oxide (NO) production. Finally, we propose that UAPC in HUVECs may play an important role in the regulation of vascular tone.
KW - Cardiomyopathy
KW - Endothelium
KW - Mechanical stimulus
KW - Muscular dystrophy
KW - Utrophin
UR - http://www.scopus.com/inward/record.url?scp=34249812937&partnerID=8YFLogxK
U2 - 10.2741/2201
DO - 10.2741/2201
M3 - Artículo
SN - 2768-6701
VL - 12
SP - 1956
EP - 1962
JO - Frontiers in Bioscience
JF - Frontiers in Bioscience
IS - 5
ER -