TY - JOUR
T1 - Evaluation of worm-bed leachate as an antifungal agent against pathogenic fungus, Colletotrichum gloeosporioides
AU - Contreras-Blancas, Eduardo
AU - Ruíz-Valdiviezo, Victor M.
AU - Santoyo-Tepole, Fortunata
AU - Luna-Guido, Marco
AU - Meza-Gordillo, Rocío
AU - Dendooven, Luc
AU - Gutiérrez-Miceli, Federico A.
N1 - Funding Information:
This project was funded by ITTG Research Internal System, project ‘Prototipo de un sistema de lombricompostaje vertical hexagonal semi-automatizado’ and Luanda farm organic products. E.C.-B. received grant-aided support from ‘Consejo Nacional de Ciencia y Tecnología’ (CONACYT, Mexico).
PY - 2014/1/2
Y1 - 2014/1/2
N2 - Worm-bed leachate is a nutrient-rich by-product of composting organic wastes with earthworms. Worm-bed leachate was stored and characterized while its possible antifungal activity towards the pathogenic fungus Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. was investigated in vitro. The pH decreased from 9.6 at the onset of the storage to 8.9 after 28 days, while the total N and C nearly halved. The surfactin concentration in the worm-bed leachate reached 10.8 μg L-1 at day 10, while that of iturin A and iturin A homologues increased towards the end of the storage. Sterilized worm-bed leachate had little effect on the growth of C. gloeosporioides, but unsterilized worm-bed leachate inhibited growth 100%. It was found that unsterilized worm-bed leachate inhibited growth of C. gloeosporioides, but sterilizing the worm-bed leachate strongly reduced the inhibitory effect indicating that the microorganisms in the worm-bed leachate out-competed the fungus for nutrients or were antagonistic towards it.
AB - Worm-bed leachate is a nutrient-rich by-product of composting organic wastes with earthworms. Worm-bed leachate was stored and characterized while its possible antifungal activity towards the pathogenic fungus Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. was investigated in vitro. The pH decreased from 9.6 at the onset of the storage to 8.9 after 28 days, while the total N and C nearly halved. The surfactin concentration in the worm-bed leachate reached 10.8 μg L-1 at day 10, while that of iturin A and iturin A homologues increased towards the end of the storage. Sterilized worm-bed leachate had little effect on the growth of C. gloeosporioides, but unsterilized worm-bed leachate inhibited growth 100%. It was found that unsterilized worm-bed leachate inhibited growth of C. gloeosporioides, but sterilizing the worm-bed leachate strongly reduced the inhibitory effect indicating that the microorganisms in the worm-bed leachate out-competed the fungus for nutrients or were antagonistic towards it.
UR - http://www.scopus.com/inward/record.url?scp=84903368356&partnerID=8YFLogxK
U2 - 10.1080/1065657X.2013.870944
DO - 10.1080/1065657X.2013.870944
M3 - Artículo
SN - 1065-657X
VL - 22
SP - 23
EP - 32
JO - Compost Science and Utilization
JF - Compost Science and Utilization
IS - 1
ER -