TY - JOUR
T1 - Evaluation of new antimicrobial agents on Bacillus spp. strains
T2 - Docking affinity and in vitro inhibition of glutamate-racemase
AU - Tamay-Cach, Feliciano
AU - Correa-Basurto, José
AU - Villa-Tanaca, Lourdes
AU - Mancilla-Percino, Teresa
AU - Juárez-Montiel, Margarita
AU - Trujillo-Ferrara, José G.
PY - 2013/10
Y1 - 2013/10
N2 - Three glutamic acid derivatives, two boron-containing and one imide-containing compound, were synthesized and tested for antimicrobial activity targeting glutamate-racemase. Antimicrobial effect was evaluated over Bacillus spp. Docking analysis shown that the test compounds bind near the active site of racemase isoforms, suggesting an allosteric effect. The boron derivatives had greater affinity than the imide derivative. In vitro assays shown good antimicrobial activity for the boron-containing compounds, and no effectiveness for the imide-containing compounds. The minimum inhibitory concentration of tetracycline, used as standard, was lower than that of the boron-containing derivatives. However, it seems that the boron-containing derivatives are more selective for bacteria. Experimental evidence suggests that the boron-containing derivatives act by inhibiting the racemase enzyme. Therefore, these test compounds probably impede the formation of the bacterial cell wall. Thus, the boron-containing glutamic acid derivatives should certainly be of interest for future studies as antimicrobial agents for Bacillus spp.
AB - Three glutamic acid derivatives, two boron-containing and one imide-containing compound, were synthesized and tested for antimicrobial activity targeting glutamate-racemase. Antimicrobial effect was evaluated over Bacillus spp. Docking analysis shown that the test compounds bind near the active site of racemase isoforms, suggesting an allosteric effect. The boron derivatives had greater affinity than the imide derivative. In vitro assays shown good antimicrobial activity for the boron-containing compounds, and no effectiveness for the imide-containing compounds. The minimum inhibitory concentration of tetracycline, used as standard, was lower than that of the boron-containing derivatives. However, it seems that the boron-containing derivatives are more selective for bacteria. Experimental evidence suggests that the boron-containing derivatives act by inhibiting the racemase enzyme. Therefore, these test compounds probably impede the formation of the bacterial cell wall. Thus, the boron-containing glutamic acid derivatives should certainly be of interest for future studies as antimicrobial agents for Bacillus spp.
KW - Boron-containing compounds
KW - Docking
KW - Glutamic acid
KW - Racemase enzyme inhibitors
UR - http://www.scopus.com/inward/record.url?scp=84884226974&partnerID=8YFLogxK
U2 - 10.3109/14756366.2012.705837
DO - 10.3109/14756366.2012.705837
M3 - Artículo
C2 - 22871135
SN - 1475-6366
VL - 28
SP - 1026
EP - 1033
JO - Journal of Enzyme Inhibition and Medicinal Chemistry
JF - Journal of Enzyme Inhibition and Medicinal Chemistry
IS - 5
ER -