TY - JOUR
T1 - Etiquetas de secuencias expresadas diferenciales de frutos de aguacate raza mexicana (Persea americana Mill. var. drymifolia)
AU - Sánchez-González, Enrique Ignacio
AU - Hernández-Delgado, Sanjuana
AU - Aguirre-Arzola, Víctor Eustorgio
AU - Torres-Castillo, Jorge Ariel
AU - Gutiérrez-Díez, Adriana
N1 - Publisher Copyright:
© 2018, Sociedade Brasileira de Fruticultura. All rights reserved.
PY - 2018
Y1 - 2018
N2 - In this study, Differential Expressed Sequence Tags (ESTs) of immature fruits of Persea americana Mill var. drymifolia of the state of Nuevo León, Mexico were developed and identified. Ten genotypes with fruits of different size and shape were selected to generate ESTs by the differential display technique. In total, 393 amplified differential fragments were obtained, 82 fragments were sequenced and edited for identification and comparison with NCBI nucleotide and protein databases. Forty sequences showed significant similarity to mRNA sequences and / or sequences of hypothetical or predicted proteins belonging to P. americana and / or other genera. Some sequences were related to enzymes such as flavanone-3-hydroxylase (F3H), lecithin-cholesterol acyltransferase, glutathione-S-transferase microsomal and pleiotropic drug resistance protein. With the information of the nucleotide composition of ESTs, primers could be designed to quantify expression levels by real-time RT-PCR of genes in different phenological stages of the fruit and to make comparisons among genotypes that allow determining alternative uses of their fruits.
AB - In this study, Differential Expressed Sequence Tags (ESTs) of immature fruits of Persea americana Mill var. drymifolia of the state of Nuevo León, Mexico were developed and identified. Ten genotypes with fruits of different size and shape were selected to generate ESTs by the differential display technique. In total, 393 amplified differential fragments were obtained, 82 fragments were sequenced and edited for identification and comparison with NCBI nucleotide and protein databases. Forty sequences showed significant similarity to mRNA sequences and / or sequences of hypothetical or predicted proteins belonging to P. americana and / or other genera. Some sequences were related to enzymes such as flavanone-3-hydroxylase (F3H), lecithin-cholesterol acyltransferase, glutathione-S-transferase microsomal and pleiotropic drug resistance protein. With the information of the nucleotide composition of ESTs, primers could be designed to quantify expression levels by real-time RT-PCR of genes in different phenological stages of the fruit and to make comparisons among genotypes that allow determining alternative uses of their fruits.
KW - Avocado
KW - Differential display
KW - EST
KW - Mexican race
UR - http://www.scopus.com/inward/record.url?scp=85042229558&partnerID=8YFLogxK
U2 - 10.1590/0100-29452018184
DO - 10.1590/0100-29452018184
M3 - Artículo
AN - SCOPUS:85042229558
SN - 0100-2945
VL - 40
JO - Revista Brasileira de Fruticultura
JF - Revista Brasileira de Fruticultura
IS - 1
M1 - e-184
ER -