Resumen
Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100®, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100® (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.
Título traducido de la contribución | Establishment of a premeabilization protocol with detergents to increase betacianine production efficiency in a beta vulgaris L. cell cultures |
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Idioma original | Español |
Páginas (desde-hasta) | 666-671 |
Número de páginas | 6 |
Publicación | Interciencia |
Volumen | 34 |
N.º | 9 |
Estado | Publicada - sep. 2009 |