Establecimiento de un protocolo de permeabilización con detergentes queincrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L.

Guadalupe Salcedo-Morales; Gabriela Trejo-Tapia; Blanca P. Martínez-Bonfil; Antonia De Jesús-Sánchez; Martha L. Arenas-Ocampo; Antonio R. Jiménez-Aparicio

Establecimiento de un protocolo de permeabilización con detergentes queincrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L.

Translated title of the contribution: Establishment of a premeabilization protocol with detergents to increase betacianine production efficiency in a beta vulgaris L. cell cultures

Guadalupe Salcedo-Morales, Gabriela Trejo-Tapia, Blanca P. Martínez-Bonfil, Antonia De Jesús-Sánchez, Martha L. Arenas-Ocampo, Antonio R. Jiménez-Aparicio

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Research output: Contribution to journal › Article › peer-review

Abstract

Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100®, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100® (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.

Translated title of the contribution	Establishment of a premeabilization protocol with detergents to increase betacianine production efficiency in a beta vulgaris L. cell cultures
Original language	Spanish
Pages (from-to)	666-671
Number of pages	6
Journal	Interciencia
Volume	34
Issue number	9
State	Published - Sep 2009

Cite this

@article{21f7ab8864234989ac42dae8381fb3ba,

title = "Establecimiento de un protocolo de permeabilizaci{\'o}n con detergentes queincrementa la eficiencia en la producci{\'o}n de betacianinas en cultivos de c{\'e}lulas de Beta vulgaris L.",

abstract = "Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100{\textregistered}, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100{\textregistered} (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.",

author = "Guadalupe Salcedo-Morales and Gabriela Trejo-Tapia and Mart{\'i}nez-Bonfil, {Blanca P.} and {De Jes{\'u}s-S{\'a}nchez}, Antonia and Arenas-Ocampo, {Martha L.} and Jim{\'e}nez-Aparicio, {Antonio R.}",

year = "2009",

month = sep,

language = "Espa{\~n}ol",

volume = "34",

pages = "666--671",

journal = "Interciencia",

issn = "0378-1844",

number = "9",

}

Establecimiento de un protocolo de permeabilización con detergentes queincrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L. / Salcedo-Morales, Guadalupe ; Trejo-Tapia, Gabriela; Martínez-Bonfil, Blanca P. et al.
In: Interciencia, Vol. 34, No. 9, 09.2009, p. 666-671.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Establecimiento de un protocolo de permeabilización con detergentes queincrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L.

AU - Salcedo-Morales, Guadalupe

AU - Trejo-Tapia, Gabriela

AU - Martínez-Bonfil, Blanca P.

AU - De Jesús-Sánchez, Antonia

AU - Arenas-Ocampo, Martha L.

AU - Jiménez-Aparicio, Antonio R.

PY - 2009/9

Y1 - 2009/9

N2 - Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100®, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100® (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.

AB - Red beet (Beta vulgaris L.) cell suspensions were permeabilized by means of four chemical detergent agents, Tween 20, 40 and 80, and Triton X-100®, to evaluate the recovery of betacianins (BC). The permeabilizating agent was selected as a function of the quantity of BC released and the contact time. Betacianin concentration was measured using digital color image analysis. The results showed that 36% of betacianins was released using Triton X-100® (0.7mM) during 10min; using these extraction conditions, the viability remained at 60-70%. This treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.

UR - http://www.scopus.com/inward/record.url?scp=73849095533&partnerID=8YFLogxK

M3 - Artículo

SN - 0378-1844

VL - 34

SP - 666

EP - 671

JO - Interciencia

JF - Interciencia

IS - 9

ER -

Establecimiento de un protocolo de permeabilización con detergentes queincrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L.

Abstract

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