TY - JOUR
T1 - Electronic and vibrational spectra of novel Lanreotide peptide capped gold nanoparticles
AU - Molina-Trinidad, E. M.
AU - Estévez-Hernández, O.
AU - Rendón, L.
AU - Garibay-Febles, V.
AU - Reguera, E.
N1 - Funding Information:
O.E.H. gratefully acknowledges a research scholarship from Mexico Government through the Ministry of Foreign Affairs. The authors are thankful to Dr. Patricia Santiago for her kindly discussion on TEM results.
PY - 2011/11
Y1 - 2011/11
N2 - Lanreotide, a somatostatin analogue peptide used for peptide receptor mediated therapy in metastatic neuroendocrine tumors, was used as capping agent of gold nanoparticles (GNPs) obtained by citrate reduction method. The displacement of the citrate groups from the GNPs surface by Lanreotide (LAN) molecules was evidenced by infrared and Raman spectra. The nanoparticles system, Au@LAN, was also characterized from HRTEM (High-Resolution Transmission Electron Microscopy) and Z-contrast images, UV-vis and EDS spectra. The stability on aging in water solution of the composite is discussed from the UV-vis spectra. The affinity constant of Au@LAN conjugate, calculated from Capillary Zone Electrophoresis data, was found to be 0.52. All the experimental evidence supports that the gold nanoparticles are effectively capped by the Lanreotide molecules through relatively strong covalent interactions. This result opens the possibility of combining the optical properties of gold nanoparticles and of Lanreotide molecule to form a bifunctional system for potential biomedical applications.
AB - Lanreotide, a somatostatin analogue peptide used for peptide receptor mediated therapy in metastatic neuroendocrine tumors, was used as capping agent of gold nanoparticles (GNPs) obtained by citrate reduction method. The displacement of the citrate groups from the GNPs surface by Lanreotide (LAN) molecules was evidenced by infrared and Raman spectra. The nanoparticles system, Au@LAN, was also characterized from HRTEM (High-Resolution Transmission Electron Microscopy) and Z-contrast images, UV-vis and EDS spectra. The stability on aging in water solution of the composite is discussed from the UV-vis spectra. The affinity constant of Au@LAN conjugate, calculated from Capillary Zone Electrophoresis data, was found to be 0.52. All the experimental evidence supports that the gold nanoparticles are effectively capped by the Lanreotide molecules through relatively strong covalent interactions. This result opens the possibility of combining the optical properties of gold nanoparticles and of Lanreotide molecule to form a bifunctional system for potential biomedical applications.
KW - Capped gold nanoparticles
KW - FTIR
KW - Lanreotide peptide
KW - Raman
KW - UV-vis
UR - http://www.scopus.com/inward/record.url?scp=80052819195&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2011.07.050
DO - 10.1016/j.saa.2011.07.050
M3 - Artículo
C2 - 21820948
SN - 1386-1425
VL - 82
SP - 283
EP - 289
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
IS - 1
ER -