Electron Microscopy and Biochemical Properties of Polyamine-Compacted DNA

Isabel Baeza; Carlos Wong; Patricio Gariglio; Luz Maria Rangel; Pedro Chavez; Lourdes Cervantes; Cecilia Montanez; Carlos Arguello

doi:10.1021/bi00394a012

Electron Microscopy and Biochemical Properties of Polyamine-Compacted DNA

Isabel Baeza, Carlos Wong, Patricio Gariglio, Luz Maria Rangel, Pedro Chavez, Lourdes Cervantes, Cecilia Montanez, Carlos Arguello

Escuela Nacional de Ciencias Biológicas (ENCB)

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

111 Citas (Scopus)

Resumen

We have obtained polyamine-compacted DNA and analyzed it by electron microscopy employing the method described by Dubochet, suitable for the study of complexes in which the main interactions are of ionic character. In addition, we have developed a simple biochemical method, based on the action of pancreatic DNase I, to demonstrate the condensation of DNA with spermidine. DNA-spermidine complexes are resistant to the action of DNase I, and there is a strong correlation between the presence of condensed DNA forms, both as toroids and as cylinders, and the insensitivity to DNase I activity. We have also shown that pBR322 DNA-spermidine complexes are transcriptionally active in the presence of Escherichia coli RNA polymerase. This supports the data concerning the biological activity of spermidine-condensed DNA.

Idioma original	Inglés
Páginas (desde-hasta)	6387-6392
Número de páginas	6
Publicación	Biochemistry
Volumen	26
N.º	20
DOI	https://doi.org/10.1021/bi00394a012
Estado	Publicada - 1987

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title = "Electron Microscopy and Biochemical Properties of Polyamine-Compacted DNA",

abstract = "We have obtained polyamine-compacted DNA and analyzed it by electron microscopy employing the method described by Dubochet, suitable for the study of complexes in which the main interactions are of ionic character. In addition, we have developed a simple biochemical method, based on the action of pancreatic DNase I, to demonstrate the condensation of DNA with spermidine. DNA-spermidine complexes are resistant to the action of DNase I, and there is a strong correlation between the presence of condensed DNA forms, both as toroids and as cylinders, and the insensitivity to DNase I activity. We have also shown that pBR322 DNA-spermidine complexes are transcriptionally active in the presence of Escherichia coli RNA polymerase. This supports the data concerning the biological activity of spermidine-condensed DNA.",

author = "Isabel Baeza and Carlos Wong and Patricio Gariglio and Rangel, {Luz Maria} and Pedro Chavez and Lourdes Cervantes and Cecilia Montanez and Carlos Arguello",

year = "1987",

doi = "10.1021/bi00394a012",

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volume = "26",

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T1 - Electron Microscopy and Biochemical Properties of Polyamine-Compacted DNA

AU - Baeza, Isabel

AU - Wong, Carlos

AU - Gariglio, Patricio

AU - Rangel, Luz Maria

AU - Chavez, Pedro

AU - Cervantes, Lourdes

AU - Montanez, Cecilia

AU - Arguello, Carlos

PY - 1987

Y1 - 1987

N2 - We have obtained polyamine-compacted DNA and analyzed it by electron microscopy employing the method described by Dubochet, suitable for the study of complexes in which the main interactions are of ionic character. In addition, we have developed a simple biochemical method, based on the action of pancreatic DNase I, to demonstrate the condensation of DNA with spermidine. DNA-spermidine complexes are resistant to the action of DNase I, and there is a strong correlation between the presence of condensed DNA forms, both as toroids and as cylinders, and the insensitivity to DNase I activity. We have also shown that pBR322 DNA-spermidine complexes are transcriptionally active in the presence of Escherichia coli RNA polymerase. This supports the data concerning the biological activity of spermidine-condensed DNA.

AB - We have obtained polyamine-compacted DNA and analyzed it by electron microscopy employing the method described by Dubochet, suitable for the study of complexes in which the main interactions are of ionic character. In addition, we have developed a simple biochemical method, based on the action of pancreatic DNase I, to demonstrate the condensation of DNA with spermidine. DNA-spermidine complexes are resistant to the action of DNase I, and there is a strong correlation between the presence of condensed DNA forms, both as toroids and as cylinders, and the insensitivity to DNase I activity. We have also shown that pBR322 DNA-spermidine complexes are transcriptionally active in the presence of Escherichia coli RNA polymerase. This supports the data concerning the biological activity of spermidine-condensed DNA.

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U2 - 10.1021/bi00394a012

DO - 10.1021/bi00394a012

M3 - Artículo

SN - 0006-2960

VL - 26

SP - 6387

EP - 6392

JO - Biochemistry

JF - Biochemistry

IS - 20

ER -

Electron Microscopy and Biochemical Properties of Polyamine-Compacted DNA

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