TY - JOUR
T1 - Effects of diets with fermented duckweed (Lemna sp.) on growth performance and gene expression in the Pacific white shrimp, Litopenaeus vannamei
AU - Flores-Miranda, Ma del Carmen
AU - Luna-González, Antonio
AU - Cortés-Espinosa, Diana Verónica
AU - Álvarez-Ruiz, Píndaro
AU - Cortés-Jacinto, Edilmar
AU - Valdez-González, Francisco Javier
AU - Escamilla-Montes, Ruth
AU - González-Ocampo, Héctor Abelardo
N1 - Publisher Copyright:
© 2014, Springer International Publishing Switzerland.
PY - 2015/4
Y1 - 2015/4
N2 - This study evaluated the effects of diets with fermented duckweed flour (Lemna sp.) (FDF) on growth performance and gene expression in Pacific white shrimp, Litopenaeus vannamei. Shrimp were cultured in an outdoor system during 50 days and fed diets containing 0, 5, 15, 25, and 35 % FDF replacing fishmeal (FM) (diets D0, D5, D15, D25, and D35, respectively). At the end of the bioassay, shrimp survival was 100 % in all treatments and growth performance was significantly better than D0 (100 % FM), especially in diet D35 with 35 % FDF. The mRNA expression of trypsin, chymotrypsin, cathepsin B, heat shock protein 70 (Lvhsp70), and heat shock protein 90 (Lvhsp90) was significantly increased at the highest FDF concentrations in diets (D15, D25, and D35) as compared to D0. Dietary FDF affected the immune system of shrimp only in diets D5 (superoxide dismutase and lysozyme) and D15 (lysozyme) where mRNA expression was significantly higher than D0. FM can be replaced with up to 35 % FDF without adversely affecting the survival and growth performance of cultured shrimp. The inclusion of FDF in diets affected the expression of stress and digestive genes, but, in immune-related genes, the effect did not show a clear trend.
AB - This study evaluated the effects of diets with fermented duckweed flour (Lemna sp.) (FDF) on growth performance and gene expression in Pacific white shrimp, Litopenaeus vannamei. Shrimp were cultured in an outdoor system during 50 days and fed diets containing 0, 5, 15, 25, and 35 % FDF replacing fishmeal (FM) (diets D0, D5, D15, D25, and D35, respectively). At the end of the bioassay, shrimp survival was 100 % in all treatments and growth performance was significantly better than D0 (100 % FM), especially in diet D35 with 35 % FDF. The mRNA expression of trypsin, chymotrypsin, cathepsin B, heat shock protein 70 (Lvhsp70), and heat shock protein 90 (Lvhsp90) was significantly increased at the highest FDF concentrations in diets (D15, D25, and D35) as compared to D0. Dietary FDF affected the immune system of shrimp only in diets D5 (superoxide dismutase and lysozyme) and D15 (lysozyme) where mRNA expression was significantly higher than D0. FM can be replaced with up to 35 % FDF without adversely affecting the survival and growth performance of cultured shrimp. The inclusion of FDF in diets affected the expression of stress and digestive genes, but, in immune-related genes, the effect did not show a clear trend.
KW - Fermented duckweed flour
KW - Fish meal
KW - Gene expression
KW - Growth performance
KW - Litopenaeus vannamei
UR - http://www.scopus.com/inward/record.url?scp=84924760273&partnerID=8YFLogxK
U2 - 10.1007/s10499-014-9835-x
DO - 10.1007/s10499-014-9835-x
M3 - Artículo
SN - 0967-6120
VL - 23
SP - 547
EP - 561
JO - Aquaculture International
JF - Aquaculture International
IS - 2
ER -