TY - JOUR
T1 - Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables
AU - Sainz-Hernández, Juan Carlos
AU - Racotta, Ilie S.
AU - Dumas, Silvie
AU - Hernández-López, Jorge
N1 - Funding Information:
We are grateful to the Plankton and Marine Biology Departments at CICIMAR-IPN for their valuable support with the equipment. The Instituto Politécnico Nacional (IPN) provided a Master's fellowship to J.C.S.H. and additional grants from COFAA-IPN and SIP-IPN for this project. This study was also supported by the CONACYT project 43249. We are also grateful to the editor at CIBNOR for modifying the English text, and to two anonymous reviewers for helpful comments.
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Eyestalk ablation is the most common procedure to induce gonadic maturation in commercial hatcheries of penaeid shrimp. In addition to reproduction, other physiological and metabolic processes are affected by removal of the X-organ sinus gland complex located in the eyestalk. In this study, the effect of unilateral and bilateral eyestalk ablation on the concentration of several hemolymph metabolites and phenoloxidase system in female and male shrimp was investigated. As a consequence of reducing or suppressing molt-inhibiting hormone (MIH) production, the duration of the molting cycle was significantly shorter in eyestalk-ablated shrimp: bilaterally (10 days), unilaterally (17 days), and shrimp that were not ablated (24 days). Mortality was significantly higher in unilaterally (35%) and bilaterally (68%) ablated shrimp than in untreated shrimp (2%), probably caused by impairment of several physiological functions mediated by hormones from the eyestalk and direct injury of the nervous system. Males and females were affected differently by eyestalk ablation in terms of concentrations of glucose, triglycerides, and protein in hemolymph. Glucose and lactate levels were lower in bilaterally ablated shrimp, as expected by the role of crustacean hyperglycemic hormone in glucose metabolism. Cholesterol and hemocyte count were not significantly different among the three treatments. Prophenoloxidase and phenoloxidase activities were significantly lower in bilaterally, but not in unilaterally ablated shrimp. This could suggest an endocrine control of this mechanism of the effector immune response or reflect the level of physiological trauma caused by bilateral eyestalk ablation in this species.
AB - Eyestalk ablation is the most common procedure to induce gonadic maturation in commercial hatcheries of penaeid shrimp. In addition to reproduction, other physiological and metabolic processes are affected by removal of the X-organ sinus gland complex located in the eyestalk. In this study, the effect of unilateral and bilateral eyestalk ablation on the concentration of several hemolymph metabolites and phenoloxidase system in female and male shrimp was investigated. As a consequence of reducing or suppressing molt-inhibiting hormone (MIH) production, the duration of the molting cycle was significantly shorter in eyestalk-ablated shrimp: bilaterally (10 days), unilaterally (17 days), and shrimp that were not ablated (24 days). Mortality was significantly higher in unilaterally (35%) and bilaterally (68%) ablated shrimp than in untreated shrimp (2%), probably caused by impairment of several physiological functions mediated by hormones from the eyestalk and direct injury of the nervous system. Males and females were affected differently by eyestalk ablation in terms of concentrations of glucose, triglycerides, and protein in hemolymph. Glucose and lactate levels were lower in bilaterally ablated shrimp, as expected by the role of crustacean hyperglycemic hormone in glucose metabolism. Cholesterol and hemocyte count were not significantly different among the three treatments. Prophenoloxidase and phenoloxidase activities were significantly lower in bilaterally, but not in unilaterally ablated shrimp. This could suggest an endocrine control of this mechanism of the effector immune response or reflect the level of physiological trauma caused by bilateral eyestalk ablation in this species.
KW - Hemolymph metabolites
KW - Phenoloxidase
KW - Shrimp
KW - Sinus gland
UR - http://www.scopus.com/inward/record.url?scp=51249088789&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2008.07.002
DO - 10.1016/j.aquaculture.2008.07.002
M3 - Artículo
SN - 0044-8486
VL - 283
SP - 188
EP - 193
JO - Aquaculture
JF - Aquaculture
IS - 1-4
ER -