TY - JOUR
T1 - Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures
AU - Tapia, Nadia
AU - Zamilpa, Alejandro
AU - Bonfill, Mercedes
AU - Ventura, Elsa
AU - Cruz-Vega, Delia
AU - Del Villar, Alma
AU - Cruz-Sosa, Francisco
AU - Osuna, Lidia
N1 - Funding Information:
Acknowledgments The authors thank Dr. Javier López Upton of the Colegio de Posgraduados-Chapingo for technical assistance in obtaining T. globosa plant material and Dr. Javier Palazón Barandela and Dra. Rosa Ma. Cusidó of the University of Barcelona for revising the manuscript. This research was supported by Fondo de Investiga-ción en Salud (FIS) Instituto Mexicano del Seguro Social (IMSS) Grant FIS/IMSS/PROT/G12/1151. PAF received a doctoral fellowship from CONACyT, México (#248629). Work in the Plant Physiology Laboratory (University of Barcelona) was financially supported by the Spanish MEC (BIO2011-29856-C02-01) and the Generalitat de Catalunya (2009SGR1217).
PY - 2013/12
Y1 - 2013/12
N2 - Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg's B5 medium supplemented with 2,4-dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg's B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg's B5) with and without the presence of methyl jasmonate (100 μM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg's B5 produced taxol (50 μg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 μg/L of taxol, 160.622 μg/L of baccatin III, 633.724 μg/L of 10-deacetyl baccatin III and 229.611 μg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg's B5, cephalomanine was obtained at a concentration of 91.428 μg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.
AB - Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg's B5 medium supplemented with 2,4-dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg's B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg's B5) with and without the presence of methyl jasmonate (100 μM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg's B5 produced taxol (50 μg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 μg/L of taxol, 160.622 μg/L of baccatin III, 633.724 μg/L of 10-deacetyl baccatin III and 229.611 μg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg's B5, cephalomanine was obtained at a concentration of 91.428 μg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.
KW - Anticancer agent
KW - In vitro cell cultures
KW - Methyl jasmonate
KW - Taxanes
KW - Taxus globosa
UR - http://www.scopus.com/inward/record.url?scp=84888097223&partnerID=8YFLogxK
U2 - 10.1007/s11738-013-1380-0
DO - 10.1007/s11738-013-1380-0
M3 - Artículo
SN - 0137-5881
VL - 35
SP - 3447
EP - 3455
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 12
ER -