TY - JOUR
T1 - Effect of repeated restraint stress on the levels of intestinal IgA in mice
AU - Jarillo-Luna, Adriana
AU - Rivera-Aguilar, Víctor
AU - Garfias, Humberto Reyna
AU - Lara-Padilla, Eleazar
AU - Kormanovsky, Alexander
AU - Campos-Rodríguez, Rafael
N1 - Funding Information:
Thanks to Allan Larsen for the skillful technical assistance. This work was supported in part by grants from CONACYT and SIP-COFAA-IPN (México).
PY - 2007/7
Y1 - 2007/7
N2 - The effects of restraint stress on the intestinal humoral immune system, particularly those about intestinal IgA production, have not been explored in detail. Thus, the purpose of this study was to assess the effect of restraint stress on the production and secretion of intestinal IgA as well as on the number of IgA+ cells in the intestinal lamina propria. The involvement of glucocorticoids and catecholamines were also evaluated. Mice were exposed to 1 or 4 h restraint stress for 4 d. The intestinal IgA concentration was quantified by ELISA and the number of IgA containing cells in the lamina propria was determined by immunohistochemistry. The effects of restraint were also analyzed in mice submitted to different procedures: adrenalectomy, chemical sympathectomy, treatment with a glucocorticoid antagonist (RU486), dexamethasone and epinephrine. The main findings were that (1) chronic restraint-stress reduced the intestinal IgA concentration without changing the number of IgA+ cells in lamina propria; (2) adrenalectomy restored the production of IgA in stressed mice; (3) RU486 and chemical sympathectomy partially blocked the decrease in intestinal IgA in stressed mice; and (4) pharmacological doses of dexamethasone and epinephrine significantly reduced the intestinal IgA concentration and the number of IgA+ cells. The restraint stress probably reduced the intestinal IgA concentration through the effects of glucocorticoids and catecholamines.
AB - The effects of restraint stress on the intestinal humoral immune system, particularly those about intestinal IgA production, have not been explored in detail. Thus, the purpose of this study was to assess the effect of restraint stress on the production and secretion of intestinal IgA as well as on the number of IgA+ cells in the intestinal lamina propria. The involvement of glucocorticoids and catecholamines were also evaluated. Mice were exposed to 1 or 4 h restraint stress for 4 d. The intestinal IgA concentration was quantified by ELISA and the number of IgA containing cells in the lamina propria was determined by immunohistochemistry. The effects of restraint were also analyzed in mice submitted to different procedures: adrenalectomy, chemical sympathectomy, treatment with a glucocorticoid antagonist (RU486), dexamethasone and epinephrine. The main findings were that (1) chronic restraint-stress reduced the intestinal IgA concentration without changing the number of IgA+ cells in lamina propria; (2) adrenalectomy restored the production of IgA in stressed mice; (3) RU486 and chemical sympathectomy partially blocked the decrease in intestinal IgA in stressed mice; and (4) pharmacological doses of dexamethasone and epinephrine significantly reduced the intestinal IgA concentration and the number of IgA+ cells. The restraint stress probably reduced the intestinal IgA concentration through the effects of glucocorticoids and catecholamines.
KW - Catecholamines
KW - Glucocorticoids
KW - IgA
KW - Intestine
KW - Plasma cells
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=34447094478&partnerID=8YFLogxK
U2 - 10.1016/j.psyneuen.2007.04.009
DO - 10.1016/j.psyneuen.2007.04.009
M3 - Artículo
C2 - 17590521
SN - 0306-4530
VL - 32
SP - 681
EP - 692
JO - Psychoneuroendocrinology
JF - Psychoneuroendocrinology
IS - 6
ER -