TY - JOUR
T1 - Effect of Heterotheca inuloides essential oil on rat cytoskeleton articular chondrocytes
AU - Martin, Denise Flores San
AU - Perea-Flores, María De Jesús
AU - Morales-López, Javier
AU - Centeno-Alvarez, Mónica María
AU - Pérez-Ishiwara, Guillermo
AU - Pérez-Hernández, Nury
AU - Pérez-Hernández, Elizabeth
N1 - Funding Information:
This study was supported by SIP-IPN projects 20120526. The authors are grateful to Departamento de Anatomía Patológica del Hospital de Ortopedia ‘Dr. Victorio de la Fuente Narváez, Distrito Federal’ for the use of equipment for cryosections.
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Osteoarthritis is characterised by progressive loss of articular cartilage through the increase of catabolic metalloproteinases, and chondrocyte cytoskeleton disruption has also been reported. In this regard, we studied the effect of Heterotheca inuloides essential oil (HIEO) on the distribution and immunolocalisation of actin, vimentin and tubulin of chondrocytes from cultured rat articular cartilage explants in the presence of the cytoskeleton disassembly agent acrylamide. After 48 h, chondrocytes treated with acrylamide showed changes in actin immunolocalisation and shrinkage, loss of tubulin compartmentalisation and vimentin collapse and redistribution. However, the immunostaining pattern of these three proteins in acrylamide- and HIEO-treated chondrocytes simultaneously retained their typical characteristics. These results suggest that HIEO promotes protein cytoskeleton reorganisation without providing a preventive effect of acrylamide-associated disassembly. However, it is also possible that HIEO prevents vimentin disorganisation by chemical interaction with acrylamide.
AB - Osteoarthritis is characterised by progressive loss of articular cartilage through the increase of catabolic metalloproteinases, and chondrocyte cytoskeleton disruption has also been reported. In this regard, we studied the effect of Heterotheca inuloides essential oil (HIEO) on the distribution and immunolocalisation of actin, vimentin and tubulin of chondrocytes from cultured rat articular cartilage explants in the presence of the cytoskeleton disassembly agent acrylamide. After 48 h, chondrocytes treated with acrylamide showed changes in actin immunolocalisation and shrinkage, loss of tubulin compartmentalisation and vimentin collapse and redistribution. However, the immunostaining pattern of these three proteins in acrylamide- and HIEO-treated chondrocytes simultaneously retained their typical characteristics. These results suggest that HIEO promotes protein cytoskeleton reorganisation without providing a preventive effect of acrylamide-associated disassembly. However, it is also possible that HIEO prevents vimentin disorganisation by chemical interaction with acrylamide.
KW - Heterotheca inuloides
KW - chondrocytes
KW - cytoskeleton
UR - http://www.scopus.com/inward/record.url?scp=84890569949&partnerID=8YFLogxK
U2 - 10.1080/14786419.2013.828289
DO - 10.1080/14786419.2013.828289
M3 - Artículo
C2 - 24088175
AN - SCOPUS:84890569949
SN - 1478-6419
VL - 27
SP - 2347
EP - 2350
JO - Natural Product Research
JF - Natural Product Research
IS - 24
ER -