TY - JOUR
T1 - Effect of entF deletion on iron acquisition and erythritol metabolism by Brucella abortus 2308
AU - Jain, Neeta
AU - Rodriguez, Araceli C.
AU - Kimsawatde, Gade
AU - Seleem, Mohamed N.
AU - Boyle, Stephen M.
AU - Sriranganathan, Nammalwar
PY - 2011/3
Y1 - 2011/3
N2 - Brucella abortus has been shown to produce two siderophores: 2,3-dihydroxybenzoic acid (2,3-DHBA) and brucebactin. Previous studies on Brucella have shown that 2,3-DHBA is associated with erythritol utilization and virulence in pregnant ruminants. The biosynthetic pathway and role of brucebactin are not known and the only gene shown to be involved so far is entF. Using cre-lox methodology, an entF mutant was created in wild-type B. abortus 2308. Compared with the wild-type strain, the ΔentF strain showed significant growth inhibition in iron minimal media that became exacerbated in the presence of an iron chelator. For the first time, we have demonstrated the death of the ΔentF strain under iron-limiting conditions in the presence of erythritol. Addition of FeCl3 restored the growth of the ΔentF strain, suggesting a significant role in iron acquisition. Further, complementation of the ΔentF strain using a plasmid containing an entF gene suggested the absence of any polar effects. In contrast, there was no significant difference in survival and growth between the ΔentF and wild-type strains grown in the murine macrophage cell line J774A.1, suggesting that an alternate iron acquisition pathway is present in Brucella when grown intracellulary.
AB - Brucella abortus has been shown to produce two siderophores: 2,3-dihydroxybenzoic acid (2,3-DHBA) and brucebactin. Previous studies on Brucella have shown that 2,3-DHBA is associated with erythritol utilization and virulence in pregnant ruminants. The biosynthetic pathway and role of brucebactin are not known and the only gene shown to be involved so far is entF. Using cre-lox methodology, an entF mutant was created in wild-type B. abortus 2308. Compared with the wild-type strain, the ΔentF strain showed significant growth inhibition in iron minimal media that became exacerbated in the presence of an iron chelator. For the first time, we have demonstrated the death of the ΔentF strain under iron-limiting conditions in the presence of erythritol. Addition of FeCl3 restored the growth of the ΔentF strain, suggesting a significant role in iron acquisition. Further, complementation of the ΔentF strain using a plasmid containing an entF gene suggested the absence of any polar effects. In contrast, there was no significant difference in survival and growth between the ΔentF and wild-type strains grown in the murine macrophage cell line J774A.1, suggesting that an alternate iron acquisition pathway is present in Brucella when grown intracellulary.
KW - Brucella
KW - Erythritol
KW - Siderophore
UR - http://www.scopus.com/inward/record.url?scp=79551474386&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2010.02186.x
DO - 10.1111/j.1574-6968.2010.02186.x
M3 - Carta
C2 - 21204922
SN - 0378-1097
VL - 316
SP - 1
EP - 6
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -